比色法测定扶正平消胶囊黄芪总皂苷的含量

目的：确定扶正平消胶囊中黄芪总皂苷的含量测定方法，并应用此方法对提取工艺初步筛方法。方法：水提，60%醇提和水提醇沉三种方法制得粗提液样品，用水饱和正丁醇萃取后，以自提黄芪甲苷为对照品。采用比色法测定其中黄芪总皂苷的含量。结果：水提，60%醇提和水提醇沉三种方法所得的样品黄芪总皂苷平均含量分别为0.26%,0.34%和0.16%。其中以60%醇提样品含量最高，结论：可应用此方法对扶正平消胶囊质量进行控制。

Determination of total astragalosides in Fuzhengpingxiao capsules by colorimetric analysis

OBJECTIVE:To establish a method to determine the content of total astragalosides in Fuzhengpingxiao capsules, and primary research about the extraction has been done with this method. METHODS: Three different methods were used to prepare the crude extractive liquid , which were extracted by pure water,60% alcohol and deposited by alcohol after extracted by pure water. The crude extractive liquid was extracted by n-butanol saturated by water. Colorimetric analysis was used to determine the content of total astragalosides. RESULTS: The mean content of total astagalosides extracted by three different methods were 0.26%,0.34% and 0.16% ,respectively. The amount of the total astragalosides extracted by 60% alcohol was the highest. CONCLUSION:The method can be used to control the quality of Fuzhengpingxiao capsules.