Enniatin A1, enniatin B1 and beauvericin on HepG2: Evaluation of toxic effects |
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| Affiliation: | 1. Section of Experimental Biomedicine, Norwegian School of Veterinary Science, Oslo, Norway;2. Mekelle University College of Veterinary Medicine, Mekelle, Ethiopia;3. Sokoine University of Agriculture, Morogoro, Tanzania;4. Institute for Global Food Security, School of Biological Sciences, Queen’s University Belfast, Northern Ireland, UK;5. Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, P.O. Box 5003, N-1432 Ås, Norway;1. Laboratory for Research on Biologically Compatible Compounds (LRSBC), Faculty of Dentistry, Rue Avicenne, 5019, Monastir, Tunisia;2. Faculty of Sciences of Bizerte, University of Carthage, 7021, Jarzouna, Tunisia;3. Laboratory of Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vincent Andrés Estelles, 46100, Burjassot, Valencia, Spain;1. Department of Pharmacology and Toxicology, University of Vienna, Althanstr. 14, A-1090 Vienna, Austria;2. Institute of Cancer Research, Department of Medicine I, Medical University of Vienna, and Comprehensive Cancer Center of the Medical University, Borschkegasse 8a, 1090 Vienna, Austria;3. Research Platform “Translational Cancer Therapy Research”, Vienna, Austria;4. Technische Universität Berlin, Institut für Chemie, Straße des 17. Juni 124, Berlin 10623, Germany |
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| Abstract: | Hepatotoxicity of three Fusarium mycotoxins, beauvericin (BEA) and two enniatins (ENNs) ENN A1 and ENN B1, in hepatocarcinoma cells (HepG2) were evaluated and compared. Concentrations used were 1.5 and 3 μM at 24, 48 and 72 h for each mycotoxin. Flow cytometry was used to examine enniatins effects on cell proliferation, to characterize the cell cycle phase where the cells blocked and to study the mitochondria role in ENNs-induced apoptosis. ENN B1 treated cells showed a time dependent G1 blockade at both concentrations used. ENN A1 and BEA decreased the apoptotic-necrotic percentage of cells comparing to control and disrupted the MMP as observed by TMRM and ToPro-3 fluorochromes signal. It is proposed a decreasing mycotoxin order by number of effects as follows: BEA > ENN B1 > ENN A1, with 47, 20 and 16%, respectively out of all situations compared. |
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| Keywords: | Mycotoxins Flow cytomtery Mitochondrial membrane potential Cell cycle Enniatins Beauvericin |
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