葛根提取物对阿尔茨海默病大鼠学习记忆能力的影响

目的:观察葛根提取物对阿尔茨海默病大鼠的学习记忆能力的影响。方法:Morris水迷宫筛选学习记忆正常SD大鼠72只,随机分为假手术组12只,手术组60只,在脑定位仪定位下,手术组大鼠微量注射凝聚态β淀粉样蛋白25-35(beta-amyloid peptide 25-35)至海马内侧脑室以建立阿尔茨海默病(AD)模型;假手术组注射等量生理盐水。Morris水迷宫筛选模型成功大鼠,随机分为模型组、0.42mg.kg-1多奈哌齐治疗组、0.47g.kg-1、0.24g.kg-1、0.12g.kg-1葛根提取物治疗组和假手术组,每组10只SD大鼠。治疗组灌胃给药,每日一次,连续四周;Morris水迷宫测试各组大鼠学习记忆能力。结果:(1)与假手术组比较,手术组大鼠学习记忆能力明显降低(P0.01)。(2)与模型组相比,多奈哌齐组、0.47 g.kg-1.d-1、0.24 g.kg-1和0.12 g.kg-1.d-1葛根提取物组均可使大鼠的逃避潜伏期变短(P0.05,P0.01),可使大鼠跨越平台次数及在平台象限游程百分比均增加(P0.05)。(3)各剂量组之间比较,于定位航行的第四、五天,大鼠逃避潜伏期差异明显(P0.05)。结论:1.大鼠侧脑室内注射Aβ25-35成功制备了大鼠AD模型。2.葛根提取物可改善AD大鼠的学习记忆能力,是否具有剂量依赖性,还需进一步研究。

The pharmacological effect of Puerarin extract on learning and memory ability of rats with Alzheimer disease

Objective： To investigate the effects of Puerarin extraction on the learning and memory ability in the rat model of Alzheime disease （AD）. Method： 72 normal SD rats were randomly divided into Sham-operated group （12 rats） and surgery group （60 rats） after being screened by Morris water maze method. 10 μg of beta-amyloid peptide 25-35 （2 μg·μL^-1） was injected into the lateral ventricle to establish AD rat model with stereotaxis instrument, and equivalent volume of saline was injected into sham operated group. Then the rats were randomly divided into sham operated group, AD model control group, 0.42 mg·kg^-1 Donepezil treated group, 0.47g·kg^-1, 0.24g·kg^-1, and 0.12g·kg^-1 Puerarin treated group （10 SD rats for each group）. The rats of Donepezil treated group were intragastric administered with Donepezil daily, and 0.47g/kg, 0.24g/kg, and 0.12g/kg Puerarin treated group were intragastric administered with Puerarin extract once a day for 4 weeks. The Morris water maze was used to investigate the ability of learning and memory of rats. Result： （1） Compared with the sham-operated group, the learning and memory ability of the rats in operation groups were decreased significantly （P 〈 0.01）. （2） Compared with the model group, 0.42 mg·kg^-1 Donepezil treated group, 0.4 7g·kg^-1, 0.24 g·kg^-1 and 0.12 g·kg^-1 Puerarin extract treated group demonstrated shortened escape latency of rats （P 〈 0.05, P 〈 0.01）, increased number of Crossing platform and run percentage of target quadrant （P 〈 0.05）.（3）Compared among the Puerarin extract treated groups, there were significant differences in the escape latency of rats, in the fourth and fifth day of the navigation test（P 〈 0.05）. Conclusion： （1） In this study, AD rat model is successfully established by injecting Aβ25-35 into lateral ventricle. （2） Puerarin extract can improve the learning and memory ability in the rat model of Alzheime disease, but urther study is still needed to explore the