大黄酸金属配合物的结构及对人肝癌HepG2细胞增殖的抑制作用

目的:以大黄酸和相应金属盐为原料,合成3种金属配合物,并对其结构进行表征,比较其抗癌活性大小。方法:采用核磁共振氢谱法、红外光谱法、紫外光谱法、滴定法、原子吸收光谱法进行结构表征;采用MTT法测试三种配合物对于人肝癌Hep G2细胞的抑制作用。结果:通过光谱法证实有配合物生成,并推测出其可能结构。MTT测试显示配合物和配体均具有一定的抗癌活性,其中大黄酸-Fe(Ⅲ)抗癌活性最强,其IC50值达17.44μg.m L-1,优于配体大黄酸(IC50=116.741μg.m L-1),大黄酸-Cu(Ⅱ)(IC50=54.427μg.m L-1),大黄酸-Cr(Ⅲ)(IC50=63.584μg.m L-1)。结论:大黄酸金属配合物抗癌活性相比配体增强。

The study on structure and inhibiting capacity of Rhein metal complex on human live tumor HepG2 cell proliferation

Objective：Synthesize three Rhein metal complex and represent the structure, then compare the antitumor activity. Method： The structures of the metal complex were studied using H-NRM spectroscopy, infrared spectroscopy, ultraviolet spectroscopy, titration and Atomic Absorption Spectroscopy. And MTT test was used to study the antitumor activity on Hep G2 cell. Result： The metal complex generation was confirmed through the structural characterization which suggested that the complex was constituted by Rhein molecular and one metal ion. The results of MTT test showed that rhein and the metal complex both had antitumor activity. Rhein-Fe（Ⅲ） had the strongest antitumor activity with the IC50 of 17.44 μg·mL^-1. And the IC50 value of Rhein, Rhein-Cu（Ⅱ）, and Rhein-Cr（Ⅲ）was 116.74 μg·mL^-1, 54.427 μg·mL^-1 and 63.584 μg·mL^-1 respectively. Conclusion： Rhein metal complexe has a stronger antitumor activity than the ligand.