坎地沙坦治疗大鼠急性胰腺炎的实验研究

目的探讨选择性血管紧张素Ⅱ受体亚型AT1拮抗剂坎地沙坦对大鼠急性胰腺炎（acute pancreatitis，AP1的影响。方法54只雄性SD大鼠随机分为正常对照组、AP组、AP＋坎地沙坦组（每组18只），腹腔注射20％L－精氨酸溶液建立AP动物模型，坎地沙坦用大鼠灌胃针灌注。各组大鼠分别在造模后第12，24，48小时分批（6只／组／批）心脏取血处死。取胰腺组织观察胰腺病理变化并评分（按Rongione标准），胰腺／体质量比，检测大鼠血清脂肪酶、TNF－α以及IL－10的变化。结果AP组胰腺炎症评分，胰腺／体质量比，血清脂肪酶、TNF－α、IL－10较对照组明显升高。AP＋坎地沙坦组胰腺炎症评分，胰腺／体质量比，血清脂肪酶以及TNF—α较AP组降低。本实验中，应用坎地沙坦对AP大鼠血清IL－10无显著影响。结论早期应用AT1受体拮抗剂坎地沙坦可以明显减轻大鼠胰腺的急性炎症病变及损伤。

Experimental research of effect of candesartan on the treatment of acute pancreatitis of rats

Objective To investigate the effects of AT1 receptor antagonist candesartan on acute pancreatitis （AP） of rats. Methods Fifty-four male Sprague Dawley rats were randomly divided into 3 groups： normal group, AP group, AP＋candesartan group, and each group contains 18 animals. AP animal models were induced by intraperitoneal injection of 20% L-arginine （2.5 g/kg）. Candesartan was given by intragastric administration （2 mg/（kg·d））. All the animals were killed in separate batches at 12 h, 24 h and 48 h （6 rats/group/ batch） post-operatively. The histopathological changes of pancreas were observed and scored （Rongione＇s scoring standard）, and pancreatic edema was evaluated. The levels of plasma lipase, TNF-α, IL-10 were measured. Results Compared with AP group, the pathologic lesions of pancreas and pancreatic edema were improved in the AP＋candesartan group, and the levels of plasma lipase, TNF-α were reduced. However, the impact of candesartan on plasma IL-10 is not significant in our experiment. Conclusion Intragastrie administration of candesartan attenuates pancreatic damage in L-arginine-induced pancreatitis of rats.