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目的观察中枢神经系统感染患儿血清和脑脊液(CSF)中基质金属蛋白酶(MMP-2、MMP-9)水平,并结合CSF白蛋白指数(AQ),探讨MMP-2、MMP-9在中枢神经系统感染血脑屏障(blood-brainbarrier,BBB)破坏中的作用。方法2004-09—2005-10河北医大附属二院采用ELISA法检测18例化脓性脑膜炎患儿急性期、恢复期及22例病毒性脑炎患儿急性期血清和CSF中MMP-2、MMP-9水平,并与非中枢神经系统感染对照组比较。结果化脓性脑膜炎(化脑)、病毒性脑炎(病脑)患儿急性期血清和CSF中MMP-2、MMP-9水平显著高于正常对照组(P<0·001),且化脑组显著高于病脑组(P<0·001)。化脑组恢复期患儿血清和CSF中MMP-2、MMP-9水平与对照组无明显差异(P>0·05)。结论中枢神经系统感染患儿血清和CSF中MMP-2、MMP-9水平显著增高,提示MMP-2、MMP-9可能参与了中枢神经系统感染的病理过程。

Levels of matrix metalloproteinases in serum and cerebrospinal-fluid in children with central nervous system infection and relationship between MMPs and impairment of blood-brain barrier

Objective To observe the levels of matrix metalloproteinases(MMPs)in serum and cerebrospinal-fluid(CSF)in children with central nervous system infection,and to combine index of CSF albumin,and to discuss the pathogenesis of MMPs in the destruction of blood-brain barrier (BBB) in the central nervous system infection.Methods The levels of MMP-2 and MMP-9 in serum and CSF were determined by ELISA in 18 children with purulent meningitis(PM) in acute phase and recovery phase,22 children with viral encephalitis(VE) in acute phase,and 20 normal children.Results In acute phase,the levels of MMP-2 and MMP-9 in serum and CSF in children with PM and VE were obviously higher than those of control group(P<0.001),and the levels of MMP-2 and MMP-9 in serum and CSF in children with PM were obviously higher than those of VE (P<0.001).In recovery phase,the difference of MMP-2 and MMP-9 in serum and CSF between PM and control group was of no statistical significance(P>0.05).Conclusion MMP-2 and MMP-9 levels of serum and CSF are significantly increased in children with central nervous system infection.MMP-2 and MMP-9 may contribute to pathologic course in BBB of central nervous system infection.