凋亡相关基因Survivin及Caspase-3 mRNA在膀胱移行细胞癌中的表达及其意义

目的探讨凋亡相关基因survivin及Caspase-3 mRNA的表达与膀胱移行细胞癌发生及发展的关系。方法应用逆转录-聚合酶链式反应（RT-PCR）检测33例膀胱移行细胞癌组织中survivin和Caspase-3 mRNA表达的情况，结合临床资料进行分析。结果93．9％（31／33）的肿瘤组织可检测到survivin mRNA表达，而对照组全部阴性表达，二者有统计学意义；81．8％（27／33）的肿瘤标本可检出Caspase-3 mRNA，而对照组中检出率为80％（8／10），实验组与对照组间无明显差异。在Ⅲ级膀胱移行细胞癌中survivin mRNA的表达强度较Ⅰ级为高，二者间有统计学意义（P〈0．05）。survivin和Caspase-3的表达与肿瘤的临床分期无关（P〉0．05）。结论survivin mRNA在膀胱移行细胞癌中有特异性表达，其高表达提示肿瘤分化不良。阻断suvivin mRNA的表达可能为膀胱肿瘤的治疗提供新的途径。

The expression of apoptosis related gene survivin and Caspase-3 mRNA in bladder transitional cell carcinoma

Obiective To evaluate the expression of survivin and Caspase-3 mRNA in the occurrence and progress of bladder transitional cell cancer （BTCC）. Methods The expressions of survivin and Caspase-3 mRNA were detected by RT- PCR and the clinical data were analyzed. Results Survivin mRNA was detected in 31 of the 33 cases of BTCC and none in the control group had detectable survivin mRNA. There was significant difference between the two groups. 27 of the 33 cases had detectable Caspase-3 mRNA, but there was no significant difference compared with the control group （81.8% vs. 80%）. Survivin mRNA had a more intense expression in grade Ⅲ than in grade Ⅰ of BTCC （P〈0.05）. The expressions of survivin and Caspase-3 mRNA were not related to the tumor stage. Conclusion Survivin mRNA expresses specifically in BTCC. The upregulation of survivin mRNA may suggest a poor differentiation of BTCC. The block of survivin mRNA may contribute a new approach to the therapy of bladder cancer.