低温对大鼠血浆及不同脑区神经肽等活性物质含量变化的影响

目的:探讨低温对血浆及不同脑区精氨酸加压素 (AVP)、血管紧张素Ⅱ (AngⅡ)、亮氨酸-脑啡肽 (L-EK)、环磷酸腺苷 (cAMP)、Ca²⁺ 及Mg²⁺含量影响。方法:复制大鼠实验性低温模型,随机分组处理,实验结束时,取下丘脑、脑干及血样品,采用放射免疫法测定AVP、AngⅡ、L-EK、cAMP的含量,原子吸收法测定Ca²⁺ 及Mg²⁺含量。结果:实验组L-EK、cAMP、Ca²⁺ 及Mg²⁺值显著高于对照组 (P <0.01);实验Ⅰ组、Ⅲ组血浆AVP、AngⅡ值显著高于对照组 (P <0.01),Ⅱ组显著低于对照组 (P <0.01)。实验Ⅰ组、Ⅲ组下丘脑和脑干AVP、AngⅡ值显著低于对照组 (P<0.01),Ⅱ组显著高于对照组 (P <0.01)。结论:低温时,L-EK、AVP、AngⅡ、cAMP、Ca²⁺ 及Mg²⁺可能参与体温调节.

Effects of hypothermia on the contents of neuropetides and other active substances in rat plasma and different brain regions

AIM: To study the effects of hypothermia on the contents of AVP,Ang Ⅱ,L-EK,cAMP,Mg²⁺and Ca²⁺in rat plasma and different brain regions.METHODS: The model of experimental hypothermia rat was established and treated in groups randomly.At the end of the experiment,samples of plasma and braintissues were taken and the contents of L-EK,cAMP,AVP,Ang Ⅱ were determined by RIA methods,Mg²⁺and Ca²⁺by atom absorption methods.RESULTS: The contents of Ang Ⅱ,AVP increased significantly in the plasma of Ⅰ,Ⅲ group(P<0.01),while that of group Ⅱ decreased obviously (P<0.01);the contents of Ang Ⅱ,AVP decreased in the hypothalamus and truncus encephalicus of Ⅰ,Ⅲ group(P<0.01),and that of group Ⅱ increased (P<0.01).CONCLUSION: L-EK,cAMP,AVP,Ang Ⅱ,Mg²⁺and Ca²⁺might be involved in body temperature regulation during hypothermia.