乳腺癌中caspase-3和caspase-9的表达及其意义

目的探讨caspase-3和caspase-9在乳腺癌发生、发展中的作用。方法采用免疫组化SP法和原位杂交(ISH)技术检测80例乳腺浸润性导管癌(invasive duct carcinoma,IDC)、23例导管内癌(ductal carcinoma in situ,DCIS)、37例增生症和9例癌旁组织中caspase-3、caspase-9 mRNA和蛋白的表达。结果 caspase-3 mRNA在各组中的表达差异无统计学意义(P>0.05)。caspase-3蛋白在IDC、DCIS、增生症中的表达均高于癌旁组织(P<0.05),在IDC、DCIS中的表达均高于增生症组(P<0.05)。caspase-9 mRNA和蛋白在IDC、DCIS、增生症中的表达均低于癌旁组织(P<0.05),IDC、DCIS中的表达均低于增生症组(P<0.05)。caspase-3、caspase-9蛋白在IDC与DCIS中的表达差异均无统计学意义(P>0.05)。caspase-3蛋白与mRNA在DCIS和增生症中的表达呈正相关(r=0.429,r=0.563,P<0.05)。caspase-9 mRNA与蛋白在各组中的表达均存在相关性(r=0.94,r=0.414,r=0.391,r=0.827,P<0.05)。caspase-3、caspase-9 mRNA和蛋白的表达与肿瘤大小等临床病理参数无相关性(P>0.05)。结论 caspase-3和caspase-9均参与乳腺导管癌的发生、发展。caspase-9蛋白表达降低,而caspase-3表达增高在乳腺癌的发生、发展过程中较常见;caspase-9表达降低可能与乳腺组织恶性转化有关。

Expression of caspase-3 and caspase-9 and their significance in breast cancer

Purpose To investigate the expression of caspase-3 and caspase-9 in breast cancer and their role in carcinogenesis and progression of breast cancer.Methods Eighty cases of breast invasive duct carcinoma(IDC),23 cases of ductal carcinoma in situ(DCIS),37 cases of adenosis and 9 cases of tumor adjacent tissues were collected.The expression of mRNA and protein of caspase-3 and caspase-9 was investigated using immunohistochemical staining and in situ hybridization.Results The expression of caspase-3 protein in IDC,DCIS and adenosis was higher than that in tumor adjacent tissues(P<0.05),and that in IDC and DCIS was higher than that in adenosis(P<0.05).No significant differences of the expression of caspase-3 mRNA were found among IDC,DCIS and tumor adjacent tissues(P>0.05).The caspase-9 protein and mRNA expression in IDC,DCIS and adenosis was lower than that in tumor adjacent tissues(P<0.05),and that in IDC and DCIS was lower than that in adenosis(P<0.05).No significant differences between the expression of caspase-3,and-9 protein were found between IDC and DCIS(P>0.05).Positive correlation was found between the expression of caspase-3 protein and mRNA in DCIS and hyperplasia(r=0.429,r=0.563,P<0.05),and between the expression of caspase-9 protein and mRNA in all groups(r=0.94,r=0.414,r=0.391,r=0.827,P<0.05).The expression of caspase-3 and-9 was unrelated to clinicopathological parameter,such as tumor size(P>0.05).Conclusions Both caspase-3 and caspase-9 are involved in carcinogenesis and progression of breast cancer,which suggests that increased expression of caspase-3 and decreased expression of caspase-9 are common events in breast cancer,and further work is needed to elucidate the exact mechanism.