RAD51基因G135C单核苷酸多态性及p53突变在肺癌中的意义

目的探讨RAD51基因G135C多态性与肺癌发病风险、病理特点及p53基因突变的相关性。方法选择80例肺癌患者为病例组,40例非肿瘤肺疾病患者为对照组。以PCR-RFLP技术检测病例组和对照组的RAD51基因型,比较不同基因型与肺癌危险性以及肺癌病理特点的关系;并采用免疫组化法对病例组进行p53突变的检测。结果 (1)RAD51基因型G/G、G/C和C/C在病例组的分布频率分别为77.5%、15.0%和1.3%,在对照组的分布频率分别为92.5%、7.5%和0。与携带G/G的个体相比,携带RAD51变异基因型(G/C和C/C)的个体具有更高的患癌风险(P<0.05);(2)RAD51基因型与肺癌的病理学类型及组织学分级无相关性;(3)RAD51 G135C基因型在不同p53基因突变状态肺癌中的分布频率差异无显著性(P>0.05)。结论 RAD51基因多态性与肺癌发病风险有关,携带RAD51变异基因型(G/C和C/C)的个体易患肺癌。

The clinical significance of RAD51 G135C polymorphysm and p53 mutation in non-small cell lung cancer

Purpose To investigate the relationship between RAD51 G135C polymorphism and lung cancer risk,pathological characteristic,and p53 mutation in lung cancer.Methods A total of 80 lung cancer cases and 40 controls with lung non-neoplastic diseases were collected.We analyzed the genotypes of RAD51(G135C) by PCR-RFLP and determined their association with lung cancer risk and pathological characteristic,and we detected the p53 mutation of 80 lung cancer cases by immunohistochemistry.Results(1)The frequency of G/G,G/C and C/C genotype was 83.7%,15.0% and 1.3% in cases and was 92.5%,7.5% and 0 in controls.When G/G served as the reference group,variant genotype(G/C and C/C) had a significantly increased risk of lung cancer(P<0.05).(2)There were no significant difference between the genotype of RAD51 and the pathological type and grade(P>0.05).(3)There were no significant difference between the distribution frequency of the genotypes of RAD51 and different p53 mutation status in lung cancer(P>0.05).Conclusion RAD51 G135C gene polymorphism may be a risk factor for lung cancer and the person who carry the variant genotype(G/C and C/C) had a significantly increased risk of lung cancer.