CISH、IHC联合检测胃腺癌EGFR、HER2基因状态及其临床意义

目的 比较显色原位杂交(CISH)与免疫组织化学(IHC)检测胃腺癌表皮生长因子(EGFR)及表皮生长因子受体2(HER2)的基因状态.方法 CISH及IHC检测85例胃腺癌组织EGFR、HER2基因扩增及蛋白表达.结果 EGFR蛋白表达阳性29例,其中表达(3+)的4例中,4例基因扩增；表达(2+)的11例中,8例基因扩增；表达(1+)的14例中,1例基因扩增.HER2蛋白表达阳性13例,其中表达(3+)的4例中,4例基因扩增；表达(2+)的6例中,5例基因扩增；表达(1+)的3例中,无基因扩增.EGFR、HER2蛋白表达及基因扩增无相关性.结论 IHC检测蛋白表达仅(3+)与CISH检测基因扩增有较好一致性,CISH检测基因状态有较好特异性.

Combined detection of EGFR and HER2 in gastric adenocarcinoma by CISH and IHC

Objective Tocomparetheexpressionsofepidermalgrowthfactor(EGFR)andhumanepidermalgrowth factor receptor 2(HER2) in gastric adenocarcinoma by chromogenic in situ hybridization(CISH) and immunohistochemistry(IHC),respectively.Methods EGFR and HER2 expressions of 85 cases with gastric adenocarcinomas were detected by CISH and IHC.Results Among 29 patients with positive EGFR,four cases with(3+) showed gene amplification;eight of 11cases with(2+) showed gene amplification;only one of the 14 cases with(1+) showed gene amplification.Among 13 patients with positive HER2,four cases with(3+) showed gene amplification;five of six cases with(2+) showed gene amplification;three cases with(1+) failed to show gene amplification.There was no correlation between gene amplification and EGFR or HER2.Conclusions Cases with(3+) of IHC is consistency with CISH preferably,and CISH has a high specificity in predicting gene status.