皮肤病理性瘢痕和瘢痕癌中连接蛋白的表达及意义

目的探讨连接蛋白Cx26、Cx32、Cx43在皮肤病理性瘢痕及瘢痕癌中的表达及意义。方法以皮肤病理性瘢痕、瘢痕癌组织为研究对象,以正常皮肤组织为对照。采用免疫组化SP法分别检测Cx26、Cx32和Cx43蛋白的表达,采用核酸分子原位杂交法检测Cx26、Cx32、Cx43 mRNA的表达,结合图像分析,分别观测3组中所检各项指标的表达(平均光密度和阳性面积),所有数据输入计算机后运用SPSS 16.0软件包进行统计学分析。结果 (1)在瘢痕癌组中,3项检测指标均呈弱阳性或阴性表达,低于病理性瘢痕组和正常皮肤组;(2)在病理性瘢痕组中,Cx26及其mRNA的表达比瘢痕癌组和正常皮肤组高;(3)Cx43及其mRNA在正常皮肤表皮、病理性瘢痕和瘢痕癌组织中的表达水平和表达强度逐渐降低,两两比较差异有统计学意义(P<0.05)。结论 (1)3种连接蛋白在瘢痕癌中低表达,可能与瘢痕癌的发生均有相关性。(2)Cx26及其mRNA在病理性瘢痕中的高表达可能参与了病理性瘢痕上皮细胞的增生。(3)与正常皮肤表皮相比较,病理性瘢痕上皮Cx43及其mR-NA的表达水平降低,可能是瘢痕癌变的危险信号。

Expression of connexins and their significance in skin pathological scar and scar carcinoma tissues

Purpose To study the expression of Cx26,Cx32 and Cx43 and their significance in skin pathological scar tissues and scar carcinoma tissues.Methods Skin pathological scar and skin scar carcinoma tissues were collected and normal skin tissues were used as control.The expression of Cx26,Cx32 and Cx43 proteins were detected by the immunohistochemical SP method,and the expression of Cx26 mRNA,Cx32 mRNA and Cx43 mRNA were detected by in situ hybridization.The expression(optical density and positive area) of every marker was measured respectively by computerized image-analysis.All data was input into the computer and statistically analyzed with SPSS 16.0 software.Results(1) The expression of Cx26,Cx32 and Cx43 showed negative or weakly positive in scar carcinoma tissues,which were lower than that in skin pathological scar group and normal skin group.(2) In the skin epithelium of pathological scar,the expression of Cx26 and its mRNA was higher than that in skin scar carcinoma group and normal skin group.(3) Cx43 and its mRNA showed negative,positive and strong positive expression in scar carcinoma tissues,skin epithelium of pathological scar and normal skin epidermis,respectively.The expression was statistically different between the any two groups(P<0.05).Conclusions(1) The low expression of Cx26,Cx32 and Cx43 proteins and its mRNA may play important roles in the pathogenesis and development in skin scar carcinoma.(2) The high expression of Cx26 and its mRNA may be related to the hyperplasia of skin pathological scar epithelium.(3) Compared with normal skin group,the low expression of Cx43 and its mRNA in the skin pathological scar may be a dangerous signal of canceration.