链霉亲和素修饰的CdSe–ZnS量子点对聚合酶链反应的影响作用初步研究

目的:探讨链霉亲和素修饰的CdSe–ZnS量子点对聚合酶链反应的影响。方法:将链酶亲和素修饰的量子点浓度进行稀释观察其对于聚合酶链反应体系的浓度效应;反应体系中含有一定浓度的链酶亲和素修饰的量子点,在不同的退火温度下进行扩增,观察链酶亲和素修饰的量子点对退火温度的影响;将不同浓度的BSA加入到含有一定浓度的链酶亲和素修饰的量子点的反应体系中,观察BSA的影响作用;将不同公司的Taq酶与不同浓度链酶亲和素修饰的量子点组合,观察对扩增体系的影响。结果:一定浓度链霉亲和素修饰的量子点可以促进聚合酶链反应的扩增效率,拓宽PCR的退火温度范围;BSA可以逆转链霉亲和素修饰的量子点对于PCR的优化作用;量子点可能是与Taq酶相互作用,来影响PCR的扩增效能。结论:一定浓度的链霉亲和素修饰的量子点可以优化PCR的反应体系。

The effects of streptavidin-modified CdSe-ZnS quantum dots on polymerase chain reaction

Objective: To explore the effects of streptavidin-modified CdSe–ZnS Quantum Dots on Polymerase Chain Reactionwere.Methods: Different concentration of the streptavidin modified CdSe–ZnS Quantum Dots were added into PCR liquids,the final PCR products were analyzed by 1 % agarose gelelectrop horesis;A certain concentration of the streptavidin modified CdSe–ZnS Quantum Dots was added into PCR liquids and amplified on different anneal temperature.Different concentration of BSA was added into PCR liquids,which has a certain concentration of the streptavidin modified CdSe–ZnS Quantum Dots,and amplified on the certain temperature;The Taq polymerase from different companies and different concentration of the streptavidin modified CdSe–ZnS Quantum Dots were respectively added into PCR liquids and amplified on the certain temperature.Results: Appropriate concentration of streptavidin modified CdSe–ZnS Quantum Dots can enhance the specificity of PCR and widen the scope of the anneal temperature;BSA can reverse the effect of streptavidin modified CdSe–ZnS quantum dots on PCR;The results implied that streptavidin Modified CdSe–ZnS Quantum Dot improved specificity of PCR by interaction with Taq polymerase. Conclusion : Appropriate concentration of streptavidin modified CdSe–ZnS Quantum Dots can improve the specificity of PCR.