锂盐对大鼠拔牙创愈合的影响

目的:观察锂盐对大鼠拔牙创愈合过程中新骨形成的影响。方法:取20只Wistar大鼠,拔除左侧上颌第二磨牙,随机分为2组。实验组从拔牙前7d起至拔牙后第3天每天腹腔注射LiCl,对照组给予相同剂量NaCl。拔牙后3d、7d处死大鼠。通过HE染色定量分析新骨的形成,BrdU标记增殖细胞。采用Image-Pro Plus生物图像分析系统对增殖细胞及新骨量作半定量分析,应用SPSS 13.0软件包对数据进行统计学分析。结果:拔牙后3d,拔牙创内间充质细胞增殖,实验组细胞增殖明显,BrdU阳性细胞数为对照组的1.8倍,两者有显著差异(P<0.05)。拔牙后7d,拔牙创底部新生骨小梁生成,相对新骨量实验组为27.0%±6.5%,对照组为12.7%±5.1%,实验组与对照组有显著差异(P<0.01)。结论:在大鼠拔牙创愈合中,前期给予锂盐可促进间充质细胞增殖,使新骨形成增加。

Effects of lithium on the healing of tooth extraction sockets in rats

PURPOSE: The purpose of this study was to investigate the effect of lithium on the healing of tooth extraction sockets in rats.METHODS: 20 Wistar rats were selected and subjected to left maxillary second molar extraction.All rats were randomly divided into two groups.The experimental group received daily LiCl injection intraperitoneally from 7 days before tooth extraction to 3 days after extraction.The control group was administrated the same volume of NaCl.All rats were sacrificed on 3 or 7 days after extraction.HE staining was carried out to investigate the new bone formation.BrdU was used to label the proliferative cells.The amount of proliferative cells and the volume of new bone were semi-quantified by Image-Pro Plus analysis system.The differences among the groups were analyzed by SPSS 13.0 software package.RESULTS: Mesenchymal cells proliferated in tooth extraction sockets on day 3 after tooth extraction.The number of BrdU labeled cells in the experimental group was 1.8 folds to that in the control group,which was of significant difference(P<0.05).On day 7,new bone formation extended from the bottom of the sockets.The volume of new trabecular bone was 27.0%±6.5% in the experimental group,which was significantly larger than 12.7%±5.1% in the control group(P<0.01).CONCLUSION: Early delivery of lithium increases the proliferation of mesenchymal cells and new bone formation in tooth extraction sockets in rats.