| 杂色曲霉素致人胚肺细胞p53及Ki-ras基因突变研究 |
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| 引用本文： | 曹文军,王会艳,张祥宏,孙旭明,谢同欣,严霞,王俊灵,王凤荣. 杂色曲霉素致人胚肺细胞p53及Ki-ras基因突变研究[J]. 卫生研究, 2000, 29(3): 175-177 |
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| 作者姓名： | 曹文军 王会艳 张祥宏 孙旭明 谢同欣 严霞 王俊灵 王凤荣 |
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| 作者单位： | 河北医科大学基础医学研究所,石家庄,050017 |
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| 基金项目： | 国家自然科学基金!( No.3 9670 813 )，河北省自然科学基金!资助项目 ( No.3 943 0 0 ) |
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| 摘 要： | 为探讨中国恶性肿瘤高发区粮食中优势污染霉菌毒素－杂色曲霉素（Ｓｔｅｒｉｇｍａｔｏｃｙｓｉｎ，ＳＴ）的致癌作用，运用银染ＰＣＲ－ＳＳＣＰ方法分析了不同浓度的ＳＴ（１μｇ／ｍｌ和３μｇ／ｍｌ）对体外培养的人胚肺细胞恶性转化过程中抑癌基因ｐ５３第５、６、７、８显外子及癌基因Ｋｉ－ｒａｓ的突变情况。结果显示ＳＴ处理后第２２周，人胚肺成纤维细胞ｐ５３基因的第８外显子和Ｋｉ－ｒａｓ癌匀出现异常泳动带型，表明Ｓ
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| 关 键 词： | 杂色曲霉素 肺肿瘤 p53 Ki－ras 基因突变 |
Mutation of p53 and Ki-ras gene in human fetal lung fibroblast cells in vitro by sterigmatocystin |
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| Cao Wenjun,Wang Huiyan,Zhang Xianghong,Sun Xuming,et al.. Mutation of p53 and Ki-ras gene in human fetal lung fibroblast cells in vitro by sterigmatocystin[J]. Journal of hygiene research, 2000, 29(3): 175-177 |
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| Authors: | Cao Wenjun Wang Huiyan Zhang Xianghong Sun Xuming et al. |
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| Affiliation: | Institute of Basic Medical Science, Hebei Medical University, Shijiazhuang 050017, China. |
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| Abstract: | To explore the carcinogenic effects of sterigmatocystin(ST), one of the predominant contaminating mycotoxins in high risk areas of cancer in China, mutation of tumor suppressor gene p53 and oncogene Ki ras in human fetal lung cells in vitro induced by ST was studied using cell culture and silver staining PCR SSCP methods. The results showed that, within 22 weeks of ST treatment, no abnormalities were found for both p53 and Ki ras gene in electrophoresis. Abnormal electrophoretic migration bands were seen at exon 8 of p53 gene and Ki ras gene in ST treated human lung fibroblast cells 22 weeks after ST treatment. Thus, the results further confirmed the carcinogenic effects of ST on human fetal lung tissue. |
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| Keywords: | sterigmatocytin lung neoplasms p53 ki ras mutation |
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