| 高效液相色谱法测定塞来昔布中三氟乙酸残留 |
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| 引用本文： | 于伟,隽海龙,王伟,史红霞.高效液相色谱法测定塞来昔布中三氟乙酸残留[J].中国实用医药,2013(36):13-14. |
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| 作者姓名： | 于伟 隽海龙 王伟 史红霞 |
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| 作者单位： | 江苏省淮安市高新技术创业中心,223001 |
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| 摘 要： | 目的建立高效液相色谱法测定塞来昔布中三氟乙酸的残留含量。方法色谱柱：采用HypersilODS柱（4．6mm×250mm，5㈣，检测波长210nm，流动相：0．08％磷酸溶液㈣3．0）-甲醇（95：5），流速1．0ml／min，柱温：35℃。结果三氟乙酸浓度在2．05～12．12μml范围内，线性关系良好，r=0．9998；检测限为1．0ng；加样回收率平均为98．57％，RSD为0．99％。结论此法可用于塞来昔布中三氟乙酸含量的测定，准确度高。
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| 关 键 词： | 塞来昔布 高效液相色谱法 三氟乙酸 含量测定 |
Determination of trifluoroacetic acid in celecoxib by HPLC |
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| Institution: | YU Wei, JUAN Hai-long, WANG Wei, et al. Huaian City High-tech Business Center, Huaian 223001, China ] |
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| Abstract: | Objective To establish a HPLC method for determination of the content of trifluoroacetic acid in celecoxib. Methods Hypersil ODS column（4.6 mm × 250 mm, 5 μm） and 0.08% phosphoric acid so -lution（ pH 3.0）-methanol（95：5） as the mobile phase at a flow rate of 1.0 ml/min were used with a column temperature of 35 ℃ and detection wavelength of 210 nm. Results The standardcurve of trifluoroacetic acid was linear in the range of 2.05-12.12μg/ml, r=- 0.9998.The detection limit was 1.0 ng. The average recovery rate of method was 98.57%, and RSD was 0.99%. Conclusion The method of content determination is practicable and accurate. It can be used for content determination of trifluoroacetic acid in celecoxib. |
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| Keywords: | Celecoxib HPLC Trifluoroacetic acid Content determination |
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