半乳糖凝集素3、细胞角蛋白19及甲状腺过氧化物酶在甲状腺乳头状癌中的表达及意义

目的 检测半乳糖凝集素3（Galectin-3）、细胞角蛋白19（CK19）和甲状腺过氧化物酶（TPO）在甲状腺腺瘤（TA）、结节性甲状腺肿伴乳头状增生（NGWPH）和甲状腺乳头状癌（PTC）中的表达状况,探讨三种抗体联合检测在甲状腺乳头状癌的早期诊断及良恶性病变鉴别诊断中的临床价值.方法 采用EliVisionTM plus两步免疫组化方法,检测Galectin-3、CK19和TPO在55例PTC、40例NGWPH和40例TA中的表达状况.结果 在55例PTC中,Galectin-3、CK19和TPO阳性率分别为92.73％、96.36％、18.18％.Galectin-3、CK19在PTC组中的阳性表达率显著高于NGWPH和TA组（P＜0.01）；而TPO在PTC组中的阳性表达率显著低于NGWPH和TA组,差异具有统计学意义（P＜0.01）；在PTC组中Galectin-3、CK19及TPO联合检测的阳性率高于NGWPH和TA组,三种抗体同时表达在PTC与甲状腺良性疾病之间的差异具有统计学意义（P＜0.01）.结论 Galectin-3、CK19和TPO联合检测更有助于PTC的准确诊断,为临床治疗提供可靠的依据.

Expression and significance of detecting Galectin-3, cytokeratin 19, thyroid peroxidase in papillary thyroid carcinoma

Objective To detect the expression of Galectin-3,cytokeratin 19 and thyroid peroxidase in thyroid adenoma （TA）,nodular goiter with papillary hyperplasia （NGWPH） and papillary thyroid carcinoma （PTC）,to explore the clinical value of three antibodies combined detection in the early diagnosis of papillary thyroid carcinoma and differential diagnosis in benign and malignant lesions.Methods EliVisionTM plus two-step method was performed to detected the expression of Galectin-3,cytokeratin 19 and thyroid peroxidase in 55 patients with PTC,40 patients with NGWPH and 40 patients with TA.Results The positive expression rates of Galectin-3,cytokeratin 19 and thyroid peroxidase in PTC were 92.73％,96.36％,18.18％,the positive expression rote in PTC significantly higher than NGWPH and TA group （P ＜ 0.01） ;The positive expression rates of thyroid peroxidase in PTC significantly lower than NGWPH and TA group （P ＜ 0.01） ; The positive expression rates of combined detection of Galectin-3,cytokeratin 19 and thyroid peroxidase in PTC significantly higher than NGWPH and TA group,simultaneously expression of three antibodies between PTC and benign thyroid diseases had a significant difference （P ＜ 0.01）.Conclusions Combined detection of Galectin-3,cytokeratin 19 and thyroid peroxidase contribute to more accurate diagnosis of PTC and provide a reliable basis for clinical treatment.