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| 免疫亲和柱净化-在线柱后光化学衍生-HPLC-FLD 同时测定甘草中黄曲霉毒素B1,B2,G1,G2 和赭曲霉毒素A的含量 | |
| 引用本文： | 韦日伟,杨小丽,仇峰,杨美华,覃洁萍.免疫亲和柱净化-在线柱后光化学衍生-HPLC-FLD 同时测定甘草中黄曲霉毒素B1,B2,G1,G2 和赭曲霉毒素A的含量[J].中国中药杂志,2011,36(17):2342-2346. |
| 作者姓名： | 韦日伟 杨小丽 仇峰 杨美华 覃洁萍 |
| 作者单位： | 1. 中国医学科学院中国协和医科大学药用植物研究所,北京100193;广西中医学院,广西南宁530001 2. 中国医学科学院中国协和医科大学药用植物研究所,北京100193;江苏大学药学院,江苏镇江212013 3. 中国医学科学院中国协和医科大学药用植物研究所,北京,100193 4. 广西中医学院,广西南宁,530001 |
| 基金项目： | 国家"重大新药创制"科技重大专项(2009ZX09502-025);国家中医药管理局中医药行业科研专项(200807042) |
| 摘 要： | 目的:建立同时检测甘草中黄曲霉毒素B1,B2,G1,G2和赭曲霉毒素A的免疫亲和柱净化-在线柱后光化学衍生-HPLC- FLD测定的方法.方法:样品经甲醇-水(80:20)超声提取后,用免疫亲和柱净化和富集;以甲醇和0.5％乙酸溶液为流动相进行梯度洗脱,通过柱后光化学衍生,荧光检测器测定.结果:黄曲霉毒素G2,G1,B2,B1和赭曲霉毒素A的检测限分别为0.02,0.06,0.015,0.03,0.25μg·kg-1,平均加样回收率为76.0％～103％,RSD低于13％.结论:该方法快速简便、准确,可用于甘草中同时测定黄曲霉毒素B1,B2,G1,G2和赭曲霉毒素A的含量. |
| 关 键 词： | 真菌毒素 甘草 免疫亲和柱 光化学衍生 HPLC 荧光检测 |
| 收稿时间： | 3/4/2011 12:00:00 AM |
Simultaneous determination of aflatoxin B1, B2, G1, G2 and ochratoxin A in Glycyrrhiza uralensis by HPLC-FLD after immunoaffinity column with online post-column photochemical derivatization |
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| WEI Riwei,YANG Xiaoli,QIU Feng,YANG Meihua and QIN Jieping.Simultaneous determination of aflatoxin B1, B2, G1, G2 and ochratoxin A in Glycyrrhiza uralensis by HPLC-FLD after immunoaffinity column with online post-column photochemical derivatization[J].China Journal of Chinese Materia Medica,2011,36(17):2342-2346. | |
| Authors: | WEI Riwei YANG Xiaoli QIU Feng YANG Meihua and QIN Jieping |
| Institution: | Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China;Guangxi Traditional Chinese Medical University, Nanning 530001, China;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China;School of Pharmacy, Jiangsu University, Zhenjiang 212013, China;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China;Guangxi Traditional Chinese Medical University, Nanning 530001, China |
| Abstract: | |
| Keywords: | mycotoxin Glycyrrhiza uralensis immunoaffinity column photochemical derivatization HPLC fluorescence detector |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |