| 巴戟天的组织培养和多倍体诱导 |
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| 引用本文： | 林美珍,巫庆珍,郑松,田惠桥.巴戟天的组织培养和多倍体诱导[J].中国中药杂志,2011,36(17):2325-2328. |
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| 作者姓名： | 林美珍 巫庆珍 郑松 田惠桥 |
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| 作者单位： | 1. 漳州卫生职业学院药学系,福建漳州,363000
2. 厦门大学生命科学学院,福建厦门,361005 |
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| 基金项目： | 福建省卫生厅医学创新课题(09-CXB-43);福建省漳州市科技计划项目(Z2008038) |
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| 摘 要： | 目的:建立巴戟天组织培养再生体系,诱导培育出巴戟天多倍体.方法:以巴戟天的幼胚为外植体诱导出愈伤组织,再用不同浓度的秋水仙素处理愈伤组织,流式细胞仪检测染色体数.结果:以秋水仙素质量浓度为500 mg·L-1处理5d效果最好,多倍体诱导率可达18.4％,诱导培育出的巴戟天多倍体根肥大增粗.结论:以巴戟天的幼胚为外植体具有消毒容易、诱导率高、分化程度低的优点；诱导出的多倍体具有一定的推广应用价值.
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| 关 键 词： | 巴戟天 幼胚 组织培养 多倍体 |
| 收稿时间： | 2011/3/27 0:00:00 |
Tissue culture andpolyploidy induction of Morinda officinalis |
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| LIN Meizhen,WU Qingzhen,ZHENG Song and TIAN Huiqiao.Tissue culture andpolyploidy induction of Morinda officinalis[J].China Journal of Chinese Materia Medica,2011,36(17):2325-2328. |
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| Authors: | LIN Meizhen WU Qingzhen ZHENG Song and TIAN Huiqiao |
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| Institution: | Department of Pharmacy, Zhangzhou Health Vocational College, Zhangzhou 363000, China;Department of Pharmacy, Zhangzhou Health Vocational College, Zhangzhou 363000, China;School of Life Sciences, Xiamen University, Xiamen 361005, China;School of Life Sciences, Xiamen University, Xiamen 361005, China |
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| Abstract: | Objective : To establish an effective protocol for plant generation and induce polyploidy of Morinda officinalis. Method : Callus was induced from immature embryo of M. officinalis and polyploidy was inducted by using colchicine treatment method. Chromosome was detected by flow cytometry. Result and Conclusion : The highest induction rate of polyploidy was 18.40%, which was obtained with 500 mg·L-1 colchicine treatment for 5 days. Roots of polyploid were bigger than diploid. Advantages of using immature embryo as explants are easy for sterilization, higher rate of callus induction and low degree dedifferentiation. The induced polyploidy of M. officinalis may have a value for spread of cultivation. |
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| Keywords: | Morinda officinalis immature embryo tissue culture polyploidy |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ |
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