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| HPLC-UV波长转换法测定玄参药材及饮片中哈巴苷与哈巴俄苷的含量 | |
| 引用本文： | 白云娥,袁鹏飞,王庆辉,王苏丽,葛跃伟,钮正睿,尚明英,刘广学,李晨,蔡少青.HPLC-UV波长转换法测定玄参药材及饮片中哈巴苷与哈巴俄苷的含量[J].中国中药杂志,2011,36(19):2697-2702. |
| 作者姓名： | 白云娥 袁鹏飞 王庆辉 王苏丽 葛跃伟 钮正睿 尚明英 刘广学 李晨 蔡少青 |
| 作者单位： | 1. 北京大学药学院生药学研究室,北京100191;山西医科大学药学院,山西太原030001 2. 北京大学药学院生药学研究室,北京,100191 3. 北京大学药学院生药学研究室,北京100191;山东中医药高等专科学校,山东莱阳265200 |
| 基金项目： | 国家药典委员会项目(YZ-016~017);国家"十一五"科技支撑计划项目(2006BAI09B05-5) |
| 摘 要： | 目的:建立同时测定中药玄参中哈巴苷与哈巴俄苷的HPLC-UV双波长含量测定方法,考察炮制对2种成分含量的影响,提出玄参药材和饮片中哈巴苷和哈巴俄苷的含量限度建议.方法:应用Agilent Technologies ZORBAX SB- C18 (4.6mm× 250 mm,5μm)色谱柱,以乙腈-0.03％磷酸水溶液为流动相,进行梯度洗脱,流速1.0 mL·min-1,柱温为25℃,采用双波长检测13 min前用210 nm,13 min以后用280 nm作为检测波长.结果:哈巴苷和哈巴俄苷能够达到很好的分离.哈巴苷线性范围为0.0549～1.46 μg;哈巴俄苷线性范围为0.022 5 ～0.900 μg.哈巴苷与哈巴俄苷平均回收率分别为98.1％,RSD2.4％(n=9);98.8％,RSD 4.3％ (n =9).10批玄参商品药材中含量哈巴苷为0.277％～0.620％,哈巴俄苷为0.078％～0.362％;10批玄参商品饮片中含量哈巴苷为0.276％～1.059％,哈巴俄苷为0.059％～0.183％;即哈巴苷平均含量玄参饮片(0.567％)高于药材(0.448％),哈巴俄苷平均含量饮片(0.128％)低于药材(0.237％).而同批次玄参药材在自制加工成饮片后哈巴苷含量值升高13.7％～ 96.0％,哈巴俄苷含量值降低11.0％～ 73.9％.结论:所建立的含量方法操作简便,结果准确,可用于玄参质量控制.玄参药材加工成饮片的过程可使哈巴苷含量值升高、哈巴俄苷含量值降低.建议玄参药材及饮片均以其中哈巴苷和哈巴俄苷总含量以干燥品计算应不低于0.45％为质量标准. |
| 关 键 词： | 玄参 哈巴苷 哈巴俄苷 高效液相色谱法 |
| 收稿时间： | 2011/1/12 0:00:00 |
Determination of harpagide and harpagoside in Scrophulariae Radix by HPLC-UV |
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| BAI Yun''e,YUAN Pengfei,WANG Qinghui,WANG Suli,GE Yuewei,NIU Zhengri,SHANG Mingying,LIU Guangxue,LI Chen and CAI Shaoqing.Determination of harpagide and harpagoside in Scrophulariae Radix by HPLC-UV[J].China Journal of Chinese Materia Medica,2011,36(19):2697-2702. | |
| Authors: | BAI Yun'e YUAN Pengfei WANG Qinghui WANG Suli GE Yuewei NIU Zhengri SHANG Mingying LIU Guangxue LI Chen and CAI Shaoqing |
| Institution: | Department of Natural Medicine, School of Pharmaceutical Science, Peking University, Beijing 100191, China;Department of Pharmacy, Shanxi Medical University, Taiyuan 030001, China;Department of Natural Medicine, School of Pharmaceutical Science, Peking University, Beijing 100191, China;Department of Natural Medicine, School of Pharmaceutical Science, Peking University, Beijing 100191, China;Department of Natural Medicine, School of Pharmaceutical Science, Peking University, Beijing 100191, China;Shandong College of Traditional Chinese Medicine, Laiyang 265200, China;Department of Natural Medicine, School of Pharmaceutical Science, Peking University, Beijing 100191, China;Department of Natural Medicine, School of Pharmaceutical Science, Peking University, Beijing 100191, China;Department of Natural Medicine, School of Pharmaceutical Science, Peking University, Beijing 100191, China;Department of Natural Medicine, School of Pharmaceutical Science, Peking University, Beijing 100191, China;Department of Natural Medicine, School of Pharmaceutical Science, Peking University, Beijing 100191, China;Department of Natural Medicine, School of Pharmaceutical Science, Peking University, Beijing 100191, China |
| Abstract: | |
| Keywords: | Scrophulariae Radix harpagide harpagoside HPLC |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |