| 超高效液相色谱-电喷雾串联质谱法快速鉴定水飞蓟中的活性成分 |
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| 引用本文： | 王奎武,胡新玲,张虹,杜琪珍. 超高效液相色谱-电喷雾串联质谱法快速鉴定水飞蓟中的活性成分[J]. 中国中药杂志, 2011, 36(17): 2361-2366 |
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| 作者姓名： | 王奎武 胡新玲 张虹 杜琪珍 |
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| 作者单位： | 1. 浙江工商大学食品与生物工程学院,浙江杭州,310035
2. 山东省新泰市第一人民医院药剂科,山东新泰,271200 |
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| 基金项目： | 国家自然科学基金项目(30973624);浙江省自然科学基金项目(Y4090121) |
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| 摘 要： | 目的:对水飞蓟中七种黄酮木脂体类活性成分在电喷雾串联质谱碎裂机制进行了探讨.方法:利用超高效液相色谱-离子肼电喷雾串联质谱(UPLC-ESI-MS/MS)与诱导碰撞解离技术(CID)在负离子模式下选择合适碰撞能量对水飞蓟中七种黄酮木脂体类活性成分进行了分析,并对其二级质谱碎裂机制进行了探讨.超高效液相色谱条件ACQUITY UPLC C18色谱柱(2.1 mm ×50 mm,1.7 μm)；流动相水-甲醇(0.1％甲酸)70∶ 30;流速0.25 mL· min-1；进样5μL.结果:超高效液相色谱可以很好地对此7种化合物进行分离,在二级质谱中异构体产生其特征碎片离子,呈现出一定的裂解规律,据此可以快速灵敏地区分此异构体.结论:本文为黄酮木脂体类化合物的快速检测提供了一种有效的方法.
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| 关 键 词： | 水飞蓟素 超高效液相色谱-串联质谱 裂解机制 |
| 收稿时间： | 2010-12-29 |
Determination of active components in silymarin by UPLC-ESIMS |
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| WANG Kuiwu,HU Xinling,ZHANG Hong and DU Qizhen. Determination of active components in silymarin by UPLC-ESIMS[J]. China Journal of Chinese Materia Medica, 2011, 36(17): 2361-2366 |
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| Authors: | WANG Kuiwu HU Xinling ZHANG Hong DU Qizhen |
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| Affiliation: | School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, China;Department of Pharmacy, Xintai First People's hospital, Xintai 271200, China;School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, China;School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, China |
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| Abstract: | A UPLC-ESIMS method was developed for simultaneous analysis of seven major bioactive flavonolignans in silymarin including silychristins A (1) and B (2), silydianin (3), silybins A (4) and B (5), and isosilybins A (6) and B (7). In this study, the seven major active flavonolignans including the diastereomers 1/2, 4/5, and 6/7 were completely separated using UPLC with an ACQUITY UPLC C18 column and MeOH-water (formic acid) mobile phase system. The collision-induced dissociation (CID) MS/MS spectra of these flavonolignans were studied systematically using ESI-MS. The results with the present methodology showed that UPLC-MS/MS can be used for general screening of active natural products from plant extracts and for the specific quality control of silymarin. |
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| Keywords: | silymarin UPLC-MS/MS fragmentation mechanism |
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