药用杭白菊和黄菊及其杂交F1代耐盐特性研究

目的:比较不同浓度NaCl处理下药用菊花红心菊和黄菊,以及二者通过正交与反交获得的F1代植株的耐盐性,分析其耐盐性的遗传机制以及耐盐性与黄酮、绿原酸等主要活性成分的相关性.方法:将材料置于加入5种不同浓度(0,40,80,120,160 mmol·L-1)NaCl的Hoagland培养液中进行胁迫处理,每日增加NaCl浓度并保证浓度梯度,一段时间后测定受伤害叶面积比率、脯氨酸、甜菜碱、丙二醛、黄酮、绿原酸的含量并分析.结果:NaCl浓度在120 mmol·L-1以下时,红心菊耐盐性高于黄菊,红心菊×黄菊耐盐性高于亲本,黄菊×红心菊表现介于亲本之间；NaCl浓度在120～ 160 mmol·L-1时,黄菊耐盐性高于红心菊,黄菊×红心菊耐盐性高于亲本而红心菊×黄菊表现介于亲本之间.结论:F1代遗传性受母本影响较多,黄酮、绿原酸含量与植株耐盐性表现出一定相关性.

Study on salt stress tolerance of Chrysanthemum morifolium 'Hangbaiju' and 'Huangju' and F1 seedlings

Objective: To study the salt stress tolerance of Hongxinju, Huangju and F₁ seedlings from orthogonal and reciprocal cross under different salt treatments. Grope for transmissibility of salt tolerance between parents and F₁ seedlings, and relativity between flavone, chlorogenic acid contents and salt tolerance. Method : The materials were put in 5 different concentrations of Hoagland nutrient solution (0, 40, 80, 120, 160 mmol·L^-1) containing NaCl, keeping grads while raising the consistency of NaCl day by day. The injured leaf area per plant, proline, betaine, MDA, flavones and chlorogenic acid contents were measured and analyzed after treatment. Result : As NaCl concentration was below 120 mmol·L^-1, the salt tolerance of Hongxinju was higher than that of Huangju, the salt tolerance of Hongxinju×Huangju higher than that of parents, the salt tolerance of Huangju×Hongxinju was at the level of parents. As NaCl concentration between 120 to 160 mmol·L^-1, the salt tolerance of Huangju was higher than that of Hongxinju, the salt tolerance of Huangju×Hongxinju higher than that of parents and the salt tolerance of Hongxinju×Huangju was at the level of parents. Conclusion : Salt tolerance of F₁ is more influenced by female parent, relativity showed between flavonoids, chlorogenic acid contents and salt tolerance.