| 应用眼底病小鼠模型须考虑的因素 |
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| 引用本文： | 庹旌生,陈之昭.应用眼底病小鼠模型须考虑的因素[J].眼科研究,2014(4):289-291. |
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| 作者姓名： | 庹旌生 陈之昭 |
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| 作者单位： | Laboratory of Immunology, National Eye Institute, NationalInstitutes of Health, Bethesda, Maryland 20892, USA |
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| 摘 要： | 随着眼科基础研究的发展,眼科疾病动物模型的应用越来越广泛,正确选择和使用合适的动物模型是合理解释眼科研究结果的保证.在眼底疾病的小鼠模型应用过程中,由于自发性和基因改造性眼底病小鼠模型来源于不同的种系,其中可能带有对所研究表型能产生干扰的基因突变.本文简单介绍最常见的4个基因的突变（变异）,以提出研究者在进行相关研究和选择动物模型时必须考虑的因素.Crb1基因、磷酸乙酰化酶6β（Pde6b）、Gnat2和RPE65基因的突变（变异）在实验小鼠中分布广泛,分别可产生rd8样视网膜变性、rd1样视网膜变性、视锥细胞功能不全和差别性光损伤敏感的表型.研究者在实验设计阶段要认真了解实验动物的遗传背景,排除有可能影响自己所研究的表型的种系.因各种条件所限确实无法避免时,也可通过设立合适的对照加以解决.
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| 关 键 词： | 模型 动物 小鼠 基因突变 视网膜疾病 视网膜变性 遗传性 表型 |
Retinal lesions and potential confounders in mouse models |
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| Institution: | Jingsheng Tuo, Chi-Chao Chan( Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda , Maryland 20892, USA) |
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| Abstract: | During the application of spontaneous or genetically engineered mouse models for retinal diseases,some phenotypes of the models might not be related to the gene （s） of interest or intentional manipulation.This problem often arises from founder effect of inbreed mouse strains.The most impact experiences in vision research community are mouse models incorporated with mutations in Crb1 （rd8）,phosphodiesterase 6 beta （Pde6b） （rd1）,Gnat2 and RPE65 genes.This paper reviewed the most relevant articles on this matter.We encourage that vision researchers who apply or develop mouse lines to study retinal diseases should carefully track and check the genetic background of their stains and/or choose proper controls in the study design. |
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| Keywords: | Model animal Mouse mutant strain Retina disease Retinal degeneration/genetics Phenotype |
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