兔直肌周围结缔组织结构观察与功能分析

背景 灵长类动物眼外肌周围的纤维弹性环结构（Pulley）与眼外肌的功能活动密切相关,但这一观点尚存在争议.观察双眼位置明显不同于灵长类动物的兔眼外肌周围结缔组织结构对分析Pully与眼球运动关系有一定的帮助. 目的 观察兔眼外肌周围结缔组织结构,分析其与以往报道的人类相同组织结构的异同,探讨这一结构与眼球运动的关系.方法 盐酸氯胺酮麻醉成年兔5只,将所有眼眶整体分离固定,每只兔任选一侧眼眶行大体解剖,另一侧眼眶行全眶冠状位连续切片,相邻切片以Weigert染色、Masson三色染色法分别进行弹性纤维和胶原纤维染色,并以小鼠抗兔α-平滑肌肌动蛋白（α-SMA）抗体标记平滑肌进行观察. 结果 胶原纤维环于兔眼球赤道部附近包绕眼外肌.胶原纤维比较菲薄且与眼直肌纤维的联系很疏松,直肌间连接带亦较菲薄.少量弹性纤维及极少量平滑肌细胞散在分布于胶原纤维中.结论 兔直肌周围结缔组织与以往报道的人类直肌周围结缔组织有明显差异,提示眼外肌周围结缔组织与眼球运动功能可能存在一定关系.

Analysis of the structure and function of the extraocular muscle connective tissue in rabbit

Background The extraocular muscles （EOMs） Pulley in primate is related to the function of EOMs,but there are arguments about that.The location of rabbit＇s eyes is different from the primate.Study on the structure of the extraocular muscle connective tissue in rabbit may play a role to analyze the function of the EOMs Pulley.Objective The structure of the connective tissue around EOMs in rabbit was studied,the difference between this structure in rabbit and homan＇ s EOMs Pulley in past reports was analyzed,and then,the role of EOMs Pulley to ocular movement was investigated.Methods Five adult rabbits were involved.The gross anatomy of an orbit in each rabbit was observed.The other orbit was processed with paraffin imbedding and coronal serial sections.A murine monocolonal antibody to α-smooth muscle actin（α-SMA） was used to show the smooth muscle,while Weigert stain was used to show muscle and collagen,and Masson trichrome stain to show the elastin.Results An encircling ring of collagen circled every EOM.The collagen ring was thin and connected to the orbital layer muscle fiber loosely.Collagen tissues coupled to adjacent EOMs.Less elastin fibers and scanty the smooth muscle cells were embedded in the collagen.Conclusions The connective tissue around rectus in rabbit is different from that in human with developed binocular ocular movements.It shows that connective tissue around EOMs may be related to the function of ocular movements.