金纳米颗粒修饰及其对肝癌细胞生长和辐射损伤的影响

目的：用巯基-聚乙二醇（SH-PEG）对金纳米颗粒（AuNPs）进行化学修饰（PEG-AuNPs）并分析其对肝癌细胞存活率和辐射对肝癌细胞作用的影响。方法制备的AuNPs用SH-PEG进行化学修饰，用透射电子显微镜观测PEG-AuNPs的大小及肝癌细胞对其的摄取，应用Cell Titer-Glo发光法和克隆形成实验分别分析PEG-AuNPs对肝癌细胞的生长抑制作用和辐射对肝癌细胞作用的影响。结果制备的PEG-AuNPs的尺寸分别为14．4 nm和30．5 nm。30．5 nm PEG-AuNPs更容易被肝癌细胞摄取，表现出明显地抑制肝癌细胞生长的作用和提高辐射对肝癌细胞的杀伤作用。结论 AuNPs经过SH-PEG化学修饰，30．5 nm PEG-AuNPs对肝癌细胞的生长抑制作用和提高辐射杀伤肝癌细胞的作用强于14．4 nm PEG-AuNPs。

Effect of Au nanoparticles modified with sulfhydryl-polyethylene glycol on liver cancer cell prolif-eration and radiation damage

Objective Au nanoparticles （AuNPs）was chemically modified with sulfhydryl-polyethylene glycol （ SH-PEG ） and its effects on liver cancer cell survival and radiation damage were assessed. Methods PEG-AuNPs were chemically fabricated with SH-PEG and AuNPs. The size of PEG-AuNPs and uptake by liver cancer cell were observed by field-emission transmission electron microscopy （TEM）. The Cell Titer-Glo luminescent cell viability assay and cell colonies were used to examine effect of PEG-AuNPs on liver cancer cells′survival and radiation damage. Results 14.4 nm and 30.5 nm PEG-AuNPs were obtained by above fabrication. 30.5 nm PEG-AuNPs were presented more absorption, inhibi-tion the survival and enhance radiation effect to liver cancer cell. Conclusions After modification with SH-PEG, larger PEG-AuNPs with 30.5 nm showed stronger inhibition in liver cancer cellular survival and promoted liver cancer cells sensitive to radiation.