丙型肝炎病毒不同编码区重组抗原在抗体检测中的应用

目的　探讨丙型肝炎病毒 (HCV)不同编码区重组蛋白片段的免疫反应性及其在抗体检测中的应用。方法　分别以 4种HCV单片段抗原 (C、NS3、NS4或NS5 )检测抗 HCV抗体阳性血清 ;以 4种单片段抗原的混合物 (MIX)检测抗 HCV抗体阳性血清、大学生体检血清和抗 HCV抗体阴性质控血清。结果　 90份抗 HCV抗体阳性血清中共有 75份可与一种或多种单片段抗原反应 ,阳性率分别为 70 .0 % (C)、6 1.1% (NS3)、5 2 .2 % (NS4 )、4 4 .4 % (NS5 ) ,其中仅与C、NS3、NS4和NS5一种抗原发生反应的分别有 8份、1份、2份和 3份血清标本 ;90份抗 HCV阳性血清经MIX抗原检测 ,阳性率为 83.3% (75 / 90 ) ;10 0份大学生血清和39份阴性质控血清经混合抗原检测均阴性。结论　HCV不同编码区重组蛋白片段有不同的免疫反应性 ,在发展抗 HCV抗体诊断试剂时 ,应采用各种不同编码区的混合抗原。

Significance of recombinant proteins encoded by different fragments of hepatitis C virus (HCV) gene for detection of anti-HCV antibodies

Objective To study the immunoreactivity of the recombinant proteins encoded by different fragments of HCV gene and explore the significance of the proteins in detection for anti-HCV antibodies.Methods Four HCV recombinant proteins encoded by C,NS3,NS4 and NS5 region respectively were applied for ELISA detection of anti-HCV antibodies.Ninety serum samples which were known to be anti-HCV-positive were tested with each of the four antigens or the mixed proteins.One hundred specimens collected from healthy undergraduates and 39 samples from an anti-HCV-negative reference panel were tested with the mixed antigens.Results Seventy-five out of the 90 known anti-HCV-positive specimens were immunoreactive to one or more of the 4 HCV recombinant antigens.The positive rates of anti-C,anti-NS3,anti-NS4 and anti-NS5 were 70.0%,61.1%,52.2% and 44.4%,respectively.Eight serum samples only reacted with C antigen.One samples only reacted with NS3 antigen.Two only reacted with NS4 antigen.One only reacted with NS5 antigen.However,all the 75 specimens (83.3%) were immunoreactive to the mixed antigens.All the specimens obtained from undergraduates and the anti-HCV-negative panel did not show any reactivity to the mixed antigens.Conclusion There is different immunoreactivity of the HCV recombinant antigens encoded by different gene fragments.The mixture of all the antigens derived from HCV different regions should be applied in further HCV diagnostic test to detect anti-HCV antibodies.