| 薏苡仁提取物体外对肾癌细胞系放射敏感性的影响及作用机制探讨 |
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| 引用本文： | 王俊杰,俞莉章,申文江,刘漓波,李亚钢,汪春林. 薏苡仁提取物体外对肾癌细胞系放射敏感性的影响及作用机制探讨[J]. 癌症, 1999, 18(6): 680-682,713 |
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| 作者姓名： | 王俊杰 俞莉章 申文江 刘漓波 李亚钢 汪春林 |
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| 作者单位： | 1. 北京医科大学第一医院肿瘤治疗中心
2. 北京医科大学第一医院泌尿外科研究所 |
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| 摘 要： | 目的：探讨康莱特（ＫＬＴ）对肾癌细胞系（ＧＲＣ－１）的放射增敏作用和机制。材料和方法：利用细胞克隆技术进行剂量－存活曲线分析，末端脱氧核苷酰转移酶法检测细胞凋亡，免疫细胞化学法分析bcl-2和ＰＣＮＡ基因表达。结果：０．２mg/mlＫＬＴ组Ｄ０值为９０．４４cＧy,空白对照组和空白乳组Ｄ０值分别为１３１．０９cＧy和１３９．４０cＧy ，统计学处理Ｐ<０．０５。０．２mg/mlＫＬＴ处理ＧＲＧ－１细胞后，ＭＬ区阳性细胞为
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| 关 键 词： | 康莱特 肾癌细胞 放射敏感性 细胞凋亡 |
| 文章编号： | 1000－467X（1999）06－0680－03 |
The study of the influence of Kang-Lai-Te injection on radiosensitivity of renal carcinoma cell lines and its mechanism |
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| WANG Jun jie,YU Li zhang,SHEN Wen jiang,et al The First Teaching Hospital of Beijing Medical University,Beijing ,P R China$$$$. The study of the influence of Kang-Lai-Te injection on radiosensitivity of renal carcinoma cell lines and its mechanism[J]. Chinese journal of cancer, 1999, 18(6): 680-682,713 |
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| Authors: | WANG Jun jie YU Li zhang SHEN Wen jiang et al The First Teaching Hospital of Beijing Medical University Beijing P R China$$$$ |
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| Affiliation: | WANG Jun jie,YU Li zhang,SHEN Wen jiang,et al The First Teaching Hospital of Beijing Medical University,Beijing 100034,P R China$$$$ |
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| Abstract: | Objective:To investigate the influence of Kang Lai Te injection (KLT) on radiosonsitivity of renal carcinoma cell lines(GRC 1) after ionizing irradiation and its mechanism Materals and Methods:Exposed to lipase 10ul/ml and 0 2 mg/ml KLT respectively for 48h,the colony formation ability of GRC 1 cells after 10mv Xray irradiation was analysed by Linear quadratic formulation The apoptotic percentages of GRC 1 cell lines were measured by TUNEL The expression of bcl 2 and PCNA genes were detected by immunohistochemistry assay Results:The D 0 values of GRC 1 cell lines were 90 44 cGy,and those of control group and lipase group were 131 09 cGy and 139 40 cGy respectively(P< 0 05),there were no significant difference between the values of lipase and control group The apoptotic percentages of GRC 1 cell lines exposed to KLT and lipase group were 89 76%and 1 02%respectively The LI of KLT and lipase group were 6 60%and 25%respective (P< 0 01) Conclusion:KLT could increase radiosensitivity of GRC 1 cell lines,induce GRC 1 cell apoptosis,down regulate expression of bcl 2 gene and up regulate expression of PCNA |
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| Keywords: | bcl-2 PCNA |
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