自发性高血压大鼠的基因组多态性

目的:应用随机扩增多态DNA聚合酶链反应（RAPD-PCR）技术分析自发性高血压大鼠（SHR）的基因组多态性。 方法:运用100条引物对WKY和SHR的DNA进行RAPD-PCR扩增，琼脂糖凝胶电泳观察。 结果:与WKY相比，100条引物中有13条引物出现基因组多态性，SHR扩增产物的电泳条带出现增加、丢失、移位和密度改变，其它87条引物扩增结果无明显改变。 结论:SHR存在基因组多态性，RAPD-PCR方法可用于检测SHR的基因组多态性。

Genomic instability of spontaneously hypertensive rats

AIM: To assess the genomic polymorphism of spontaneously hypertensive rats(SHR) by using random amplified polymorphic DNA PCR(RAPD-PCR).METHODS: The DNA of WKY rats and SHR was amplified with RAPD-PCR using 100 single primers.The PCR products were resolved with electrophoresis on agarose gel and stained with ethidium bromide.RESULTS: Thirteen of the 100 single primers resulted in genomic polymorphism.In the RAPD method,genetic alterations appeared as either loss or gain of a band,shift of a band,or decrease or increase in intensities of a band of SHR DNA relative to WKY rat tissue DNA.The other primers produced amplification products were not different in two groups.CONCLUSION: It is suggested that SHR present genomic polymorphism.RAPD-PCR is an effective technique to detect genomic polymorphism in SHR.