| 当归多糖对小鼠骨髓基质细胞黏附分子表达和黏附功能的影响 |
| |
| 引用本文： | 王改琴,吴宏.当归多糖对小鼠骨髓基质细胞黏附分子表达和黏附功能的影响[J].第四军医大学学报,2009,30(1):53-56. |
| |
| 作者姓名： | 王改琴 吴宏 |
| |
| 作者单位： | 重庆医科大学干细胞与组织工程研究室,组胚教研室,重庆,400016
|
| |
| 摘 要： | 目的：研究当归多糖（APS）对小鼠骨髓基质细胞（BMSCs）生长、粘附分子ICAM-1和VCAM-1表达及其对骨髓单个核细胞黏附功能的影响．方法：观察APS对小鼠BMSCs消化时间的影响；MTT法检测APS对小鼠BMSCs增殖及对骨髓单个核细胞黏附功能的影响；流式细胞术检测APS对BM—SCs表面ICAM-1和VCAM-1表达的影响．结果：100，200mg／LAPS使BMSCs达80％贴壁后消化时间明显缩短（P〈0．05）；50，100mg／LAPS组BMSCs增殖能力较对照组增强（P〈0．05）；200，300mg／LAPS能使BMSCs表面ICAM-1表达降低，而50，100，200，300mg／LAPS均可使VCAM-1表达降低（P〈0．05）；体内注射APS后BMSCs表面ICAM-1和VCAM-1表达均降低（P〈0．05）；100，200，300mg／LAPS使小鼠BM．SCs对单个核细胞黏附率减弱（P〈0．05）．结论：APS能促进小鼠BMSCs增殖，降低其表面黏附分子ICAM-1和VCAM-1的表达，进而使小鼠BMSCs达80％贴壁后消化时间明显缩短，对骨髓单个核细胞的黏附率减弱．
|
| 关 键 词： | 当归多糖 骨髓基质细胞 黏附分子 VCAM-1 ICAM-1 |
Effects of angelica polysaccharides on expression of adhesion molecules and adhesive function of murine bone marrow stromal cells |
| |
| WANG Gai-Qin,WU HongLaboratory of stem cell , tissue engineering.Effects of angelica polysaccharides on expression of adhesion molecules and adhesive function of murine bone marrow stromal cells[J].Journal of the Fourth Military Medical University,2009,30(1):53-56. |
| |
| Authors: | WANG Gai-Qin WU HongLaboratory of stem cell tissue engineering |
| |
| Institution: | WANG Gai-Qin,WU HongLaboratory of stem cell and tissue engineering,Department of Histology and Embryology,Chongqing Medical University,Chongqing 400016,China |
| |
| Abstract: | AIM:To explore the effects of angelica polysaccharides(APS) on the cell growth, the expression of intercellular adhesion molecule-1(ICAM-1) and vascular cell adhesion molecule -1(VCAM-1) in murine bone marrow stromal cells (BMSCs) and the adhesive rate of murine bone marrow mononuclear cells to BMSCs. METHODS:The effects of APS on the digestion time of the murine BMSCs were observed with inverted microscope. The proliferation of BMSCs and the binding capacity of murine bone marrow mononuclear cells to BMSCs... |
| |
| Keywords: | VCAM-1 ICAM-1 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
