程序性细胞死亡在小鼠腭突发育及腭裂形成中的意义

目的:明确程序性细胞死亡在腭裂形成中的作用。进一步探讨调控程序性细胞死亡的相关基因在腭裂形成中的变化及分子生物学机制。方法:将16只妊娠10d(GD10)的C57BL/6N系小鼠随机分为实验组和对照组。实验组管饲维甲酸80mg/kg,对照组管饲等体积植物油。分别于GD1314(妊娠第13天第14小时,下同),GD1322,GD148,GD1414,GD1422,GD158,GD1522,GD168d处死孕鼠取胚鼠。胚鼠头部切片做原位末端转移酶标记(TUNEL)染色。结果:在腭突垂直生长期及定向移动期,两组腭间质细胞发生程序性死亡的数量有明显的差异(P<0.05)。结论:经外源性维甲酸作用后的小鼠腭突间质细胞发生大量程序性死亡,腭突体积发育瘦小,未能在中线相互融合而导致腭裂。

Significance of Programmed Cell Death in C57BL/6N;Strain Mouse Palate Process Development and Cleft Palate Formation

Objective:To observe the changes of programmed cell death (PCD) in C57BL/6N strain mouse cleft palate formation and study related gene of PCD in future.Methods: 16 mice (gestation of 10 days,GD10) were divided into the experimental and control groups randomly,then the mice of the experimental group were dosed with retinoic acid(RA) 80 mg/kg,and the tissue slices of the embryo mouse were prepared for TdT mediated dUTP nick end labeling (TUNEL)staining at GD13 {14}(the 14th hour of the 13th day gestation), GD13 {22},GD14 8,GD14 {14},GD14 {22},GD15 8,GD15 {22} and GD16 8 respectively.Results: In the early development of palate process, the positive index of PCD was significantly higher in the experimental group than in the control group (P<0 05).Conclusion:The effects of RA on mesenchymal cells of the early development of palate process in the experimental group induce PCD, which result in the formation of small palate process and failure of fusing of the shelves.;