Antigenicity of fractions ofHelicobacter pylori prepared by fast protein liquid chromatography and urease captured by monoclonal antibodies |
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| Authors: | A R Stacey P R Hawtin D G Newell |
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| Institution: | (1) Pathology Division, Public Health Laboratory Service Centre for Applied Microbiology and Research, Porton Down, SP4 OJG Salisbury, UK;(2) Public Health Laboratory Service, Southampton General Hospital, SO9 4XY Southampton, UK |
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| Abstract: | The antigenicity ofHelicobacter pylori protein fractions separated by fast protein liquid chromatography size exclusion was investigated by EIA with sera from patients of well definedHelicobacter pylori status. The antigenic material ofHelicobacter pylori was confined to fractions 8 and 14 to 21. Urease containing fractions (14/15) and flagella containing fractions (17/18) were identified. Fraction 8 non-specifically bound human immunoglobulin as demonstrated by the binding ofHelicobacter pylori negative sera. The remaining fractions 14 to 21 when used individually as EIA antigens were 91–100 % specific, however fractions 16 to 19 showed a reduced sensitivity (78 %) compared with the acid extract (95 %). The urease fractions were 91 % sensitive. Purified urease antigen captured by antiurease monoclonal antibodies was 83 % sensitive and 93.3 % specific. |
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