肝癌微环境中CD4+CD25+调节性T细胞与T细胞免疫的关系

目的 了解肝细胞癌组织CD4 CD25 调节性T细胞(以下简写Treg)与肿瘤微环境T细胞免疫的关系.方法 对52例肝癌组织和癌旁组织用CD4、CD25双重酶标免疫组化染色和用CD8En Vision法染色,对癌组织中Treg细胞和CD4 T、CD8 T、CD4 T/CD8 T比值进行相关性分析.结果 肝癌以及癌旁组织中Treg细胞单个高倍视野平均数分别为7.6308±2.8368、5.1654±1.6718;两组比较有显著差异,P=0.000;肝癌组织中Treg细胞的数量与其浸润性CD4 T淋巴细胞的数量以及CD4 T/CD8 T比值呈显著负相关,r=-0.538,P=0.014;r=-0.545,P=0.000,与浸润性CD8 T淋巴细胞的数量分布无明显相关性,r=-0.403,P=0.078.结论 Treg在肝癌微环境中可能通过细胞接触的方式抑制CD4 T淋巴细胞的增殖来抑制肿瘤局部免疫,使肿瘤细胞逃避免疫监视.因此除去或减少肝癌微环境中的Treg细胞有利于提高肿瘤的免疫治疗效果.

The relationship between CD4+CD25+ regulatory T Cells and T lymphocytes immunity in tumor microenvironment of hepatocellular carcinoma

Objective To investigate the relationship between CD4 CD25 regulatory T cells and T lymphocytes immunity in tumor microenvironment.Methods we marked CD4 CD25 Treg and CD4 T cells in the slides tissues using Double-immunohistochemical,and marked CD8 Tcells in the slides tissues using En Vision.Results The number of Treg cell in the tissues of HCC was more than that of in the tissue of peri-cancer,with significant statistical difference(P=0.000).The ratio of CD4 T/CD8 T and CD4 T cell infiltration was positive relation to the number of Treg cell in HCC tissues(r=-0.539,P=0.014,r=-0.545,P=0.000);however,CD8 T cell infiltration was no relation to the number of Treg cell in HCC tissues(r=-0.403,P=0.078).Conclusion Treg cells is the main immunosupression cell in tumor microenvironment of HCC,Treg cells suppress proliferation of CD4 T cell in a contact-dependent manner possibly so that tumor cells can escape immune surveillance and result in invasion and progression.Functional deletion of tumor-infiltrating Treg cells could enhance tumor-specific immunotherapy.