| 黑节草茎段直接诱导丛生芽 |
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| 引用本文： | 王进红,张雪梅,付开聪. 黑节草茎段直接诱导丛生芽[J]. 时珍国医国药, 2000, 11(11): 1052-1052 |
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| 作者姓名： | 王进红 张雪梅 付开聪 |
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| 作者单位： | 云南思茅地区民族传统医药研究所,665000 |
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| 摘 要： | 介绍利用黑节草茎节外殖体获得丛生苗的方法：黑节草茎节段在１／２ｍｓ＋６－ＢＡ２ｍｇ／Ｌ＋ＮＡＡ０．２ｍｇ／Ｌ的培养基上，先诱导形成愈伤组织，然后出许多绿色小芽。进一步移至１／２ｍｓ＋ＮＡＡ０．５ｍｇ／Ｌ培养基上产生大量的丛生芽。丛生芽培养于１／２ｍｓ＋ＩＢＡ０．５ｍｇ／ｌ＋ＮＡＡ０．５ｍｇ／Ｌ培养基上，可诱导生根形成完整植株。
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| 关 键 词： | 黑节草 组织培养 植株再生 丛生芽 茎段 |
Rosette Bud Induction from Stem Segments of Dendrobium candidun |
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| WANG Jin-hong,ZHANG Xue-mei,FU Kai-cong. Rosette Bud Induction from Stem Segments of Dendrobium candidun[J]. Lishizhen Medicine and Materia Medica Research, 2000, 11(11): 1052-1052 |
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| Authors: | WANG Jin-hong ZHANG Xue-mei FU Kai-cong |
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| Abstract: | Callus was induced on modified ms medium supplemented with 6-BA 2 mg/L and NAA 0.2 mg/L from the stem segments of Dendrobium candidun. Thereafter a substantial amount of green bud apices were produced. When they were transplanted into 1/2 ms with NAA 0.5 mg/L basic medium, a large amount of rosette buds were formed. Roots were induced on modified 1/2 ms with NAA 0.5 mg/L medium supplemented with IBA 0.5 mg/L and NAA 0.5 mg/L and intact plantlets were thus regenerated. |
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| Keywords: | Dendrobium Tissue culture Plantlet regenevation |
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