HPLC法同时测定蒙药菟丝草中3种成分的含量

目的:建立同时测定蒙药菟丝草中槲皮素、金丝桃苷和山柰酚3种成分含量的方法。方法:采用高效液相色谱法。色谱柱为Hypersil ODS-2(300 mm×4.6 mm,5μm),流动相为甲醇-水(58∶42,V/V),检测波长为281 nm,柱温为30℃,流速为1.0 ml/min。结果:槲皮素、金丝桃苷和山柰酚的质量浓度分别在10⁵0、2.550、2.5³0、2.530、2.5³0μg/ml范围内与各自峰面积积分值呈良好的线性关系(r分别为0.999 7、0.999 5、0.999 6);精密度、稳定性、重复性试验的RSD<2%;平均加样回收率分别为98.90%、97.60%、97.39%,RSD分别为1.23%、1.37%、1.40%(n均为6)。结论:该方法简便、快速、准确,可为蒙药菟丝草的质量评价提供依据。

Simultaneous Determination of 3 Components in Cuscuta chinensis by HPLC

OBJECTIVE：To establish a method for simultaneous determination of quercetin, hyperoside and kaempferol from Cuscuta chinensis. METHODS： HPLC method was adopted. The separation was performed on Hypersil ODS-2 （300 mmx4.6 mm, 5 gm） column with mobile phase consisted of methanol-water （58：42, V/V）. The detection wavelength was set at 281 nm and column temperature was 30 ℃. The flow rate was 1.0 ml/min. RESULTS ： The linear ranges of quercetin, hyperoside and kaempferol were 10-50 μg/ml（r=0.999 7）,2.5-30 μg/ml（r=0.999 5）,2.5-30μg/ml（r=0.999 6）,respectively. RSDs of precision, stability and reproducibility tests were all lower than 2 %. The average recoveries of three active compounds were 98.90% （RSD= 1.23 %, n=6）, 97.60% （RSD=1.37%, n=6） and 97.39% （RSD=I.40% ,n=6）. CONCLUSIONS： The method is simple, rapid and accurate, and provides reference for quality evaluation of C. chinensis.