| 柑柏止痒浴液的质量标准研究 |
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| 引用本文： | 余晓霞,;伍俊妍,;刘春霞,;温预关. 柑柏止痒浴液的质量标准研究[J]. 中国药房, 2014, 0(31): 2939-2941 |
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| 作者姓名： | 余晓霞, 伍俊妍, 刘春霞, 温预关 |
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| 作者单位： | [1]中山大学孙逸仙纪念医院药学部,广州510120; [2]广州市脑科医院药剂科/国家药品临床研究基地,广州I510370 |
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| 摘 要： | 目的:建立柑柏止痒浴液的质量标准。方法:采用薄层色谱(TLC)法对处方中薄荷脑和黄柏进行定性鉴别;采用高效液相色谱法测定黄柏中盐酸小檗碱的含量:色谱柱为Agilent Eclipse Plus C18(100 mm×4.6 mm,5μm),流动相为甲醇-0.03 mol/ml乙酸铵溶液(45∶55,V/V),检测波长为346 nm。结果:TLC图专属性强,阴性对照无干扰。盐酸小檗碱的质量浓度在1200μg/ml范围内与峰面积积分值呈良好的线性关系(r=0.999 8);精密度、稳定性、重复性试验的RSD<2%;平均加样回收率为100.28%,RSD=1.58%(n=6)。结论:所建标准可用于柑柏止痒浴液的质量控制。
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| 关 键 词： | 柑柏止痒浴液 质量标准 薄层色谱法 高效液相色谱法 盐酸小檗碱 |
Study on Quality Standard of Ganbai Zhiyang Lotion |
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| Affiliation: | YU Xiao-xia, WU Jun-yan, LIU Chun-xia, WEN Yu-guan( 1.Dept. of Pharmacy, Sun Yat-sen Memorial Hospi- tal, Sun Yat-sen University, Guangzhou 510120, China; 2.Dept. of Pharmacy, Guangzhou Brain Hospital/State Clinical Drug Research Base, Guangzhou 510370,China) |
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| Abstract: | OBJECTIVE： To establish the quality standard of Ganbai zhiyang lotion. METHODS： Menthol and Phellodendron chinense were identified by TLC. The content of berberine hydrochloride in P chinense was determined by HPLC. The determination was performed on Agilent Eclipse Plus C18 （100 mm×4.6mm,5μm） column with mobile phase consisted of methanol-0.03 mol/ml ammonium acetate（45： 55, V/V）. The detection wavelength was set at 346 nm. RESULTS： TLC identification method exhibited good specificities without interference from negative samples. The linear range berberine hydrochloride were 1-200 μg/ml（r= 0.999 8） ; RSDs of precision, stability and reproducibility tests were all lower than 2% ; average recovery was 100.28% （RSD= 1.58% ,n=6）. CONCLUSIONS： The established standard can be used for the quality control of Ganbai zhiyang lotion. |
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| Keywords: | Ganbai zhiyang lotion Quality standard TLC HPLC Berberine hydrochloride |
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