| HPLC法测定荜茇根中胡椒碱的含量 |
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| 引用本文： | 胡玉,吕顺忠,高鸿亮,王建华,罗兰,姚萍. HPLC法测定荜茇根中胡椒碱的含量[J]. 中国药房, 2014, 0(7): 633-635 |
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| 作者姓名： | 胡玉 吕顺忠 高鸿亮 王建华 罗兰 姚萍 |
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| 作者单位： | [1]新疆医科大学基础医学院,乌鲁木齐830054 [2]新疆医科大学第一附属医院药学部,乌鲁木齐830054 [3]新疆医科大学第一附属医院消化科,乌鲁木齐830054 |
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| 基金项目： | 国家自然科学基金资助项目（No.81260670） |
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| 摘 要： | 目的:建立测定荜茇根中胡椒碱含量的方法。方法:采用高效液相色谱法。色谱柱为Inertsil ODS-3(250 mm×4.6 mm,5μm),流动相为甲醇-水(77∶23,V/V),检测波长为343 nm,流速为1.0 ml/min,柱温为30℃。结果:胡椒碱的质量浓度在0.000 80.024 0 mg/ml范围内与其峰面积积分值呈良好的线性关系(r=0.999 9);精密度、稳定性、重复性试验的RSD<2%;平均加样回收率为100.85%,RSD=0.82%(n=6)。结论:该方法简便、快速、准确、重复性好,可用于荜茇药材的质量控制。
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| 关 键 词： | 高效液相色谱法 荜茇根 胡椒碱 含量测定 |
Content Determination of Piperine in the Roots of Piper longum by HPLC |
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| HU Yu,LYU Shun-zhong,GAO Hong-liang,WANG Jian-hua,LUO Lan,YAO Ping. Content Determination of Piperine in the Roots of Piper longum by HPLC[J]. China Pharmacy, 2014, 0(7): 633-635 |
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| Authors: | HU Yu LYU Shun-zhong GAO Hong-liang WANG Jian-hua LUO Lan YAO Ping |
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| Affiliation: | 3 ( 1.College of Basic Medicine, Xinjiang Medical University, Urumqi 830054, China; 2. Dept. of Pharmacy, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China; 3. Dept. of Gastroentrology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China) |
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| Abstract: | OBJECTIVE： To establish a method for simultaneous determination of piperine in the roots of Piper longum. METHODS： HPLC method was adopted. The determination was performed on Inertsil ODS-3 （250 mm ×4.6 mm, 5 μm） column with mobile phase consisted of methanol-water （77 ： 23, V/V） at the flow rate of 1.0 ml/min. The detection wavelength was set at 343 nm, and column temperature was 30 ℃. RESULTS： The linear range of piperine were 0.000 8-0.024 0 mg/ml（r=0.999 9）; RSDs of precision, stability and reproducibility tests were lower than 2% ; average recovery was 100.85% （RSD=0.82%, n=6）. CONCLUSIONS： The method is simple, rapid, accurate and reproducible, and can be used for quality control for the roots of P longum. |
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| Keywords: | HPLC The roots of Piper longum Piperine Content determination |
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