一测多评法测定一清颗粒中5种蒽醌类成分的含量

目的:建立一清颗粒中5种主要活性成分的一测多评含量测定方法。方法:以大黄素为内标物,采用高效液相色谱法,建立其与其他4种成分芦荟大黄素、大黄酚、大黄素甲醚、大黄酸的相对校正因子,并用该校正因子进行计算,实现一测多评;同时采用外标法对5种成分进行含量测定,通过比较两法结果验证该方法的准确性。结果:建立的校正因子重复性较好,采用校正因子计算的含量值与外标法实测值偏差较小。结论:采用一测多评法测定一清颗粒中5种蒽醌类成分的含量是可行的。

Content Determination of 5 Anthraquinones in Yiqing Granules by Multi-components Quantitation by One Marker

OBJECTIVE： To establish the multi-components quantitation by one mark for content determination of 5 anthraquinones in Yiqing granules. METHODS： Using emodin as internal substance, HPLC method was adopted to determination the content of emodin and obtain relative correction factor of it to other 4 components for calculation. The multi-components quantitation by one mark was conducted. The external standard method was adopted for the content determination of 5 components and compared with multi-components quantitation by one mark. RESULTS： The relative correction factor showed good reproducibility; there was slight difference betweSn calculated value by correction factor and measured value＇of external standard method. CONCLUSIONS： The method is feasible for the content determination of 5 anthraquinones in Yiqing granules.