| HPLC法测定辽源七厘复方中药控释微丸中龙血素B的含量 |
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| 引用本文： | 刘应杰,;杨宗发,;何文生,;江尚飞,;谭小梅,;何静,;邱妍川.HPLC法测定辽源七厘复方中药控释微丸中龙血素B的含量[J].中国药房,2014(31):2929-2930. |
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| 作者姓名： | 刘应杰 ;杨宗发 ;何文生 ;江尚飞 ;谭小梅 ;何静 ;邱妍川 |
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| 作者单位： | [1]1重庆医药高等专科学校药学院,重庆401331; [2]重庆市大足区人民医院药剂科,重庆402360 |
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| 基金项目： | 重庆市卫生局中医药科研计划立项（No.2011-2-171） |
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| 摘 要： | 目的:改良测定辽源七厘复方中药控释微丸中龙血素B含量的方法。方法:采用高效液相色谱法。色谱柱为Kromasil C18(250 mm×4.6 mm,5μm),流动相为甲醇-1%冰醋酸溶液(梯度洗脱),流速为1.0 ml/min,检测波长为206 nm。结果:龙血素B的质量浓度在0.4020.00μg/ml范围内与峰面积积分值呈良好线性关系(r=0.999 5);精密度、稳定性、重复性试验的RSD<2%;平均加样回收率为100.50%,RSD=1.90%(n=6)。结论:该改良方法操作简便、分离效果好、灵敏度高、重复性好,可用于辽源七厘复方中药控释微丸的质量控制。
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| 关 键 词： | 辽源七厘复方中药控释微丸 龙血素B 高效液相色谱法 含量测定 |
Content Determination of Loureirin B in Liaoyuan Qili Compound TCM Controlled-release Pellets by HPLC |
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| Institution: | LIU Ying-jie, YANG Zong-fa, HE Wen-sheng, JIANG Shang-fei, TAN Xiao-mei, HE Jing, QIU Yan-chuan( 1. College of Pharmacy, Chongqing Medical and Pharmaceutical College, Chongqing 401331, China; 2.Dept of Phar- macy, Chongqing Dazu District People＇ s Hospital, Chongqing 402360, China) |
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| Abstract: | OBJECTIVE ： To improve the method for content determination of loureirin B in Liaoyuan qili compound TCM controlled-release pellets. METHODS： HPLC method was adopted. The determination was carded out on Kromasil C18 （250 mm×4.6mm,5μm） column with mobile phase consisted of methanol-1% glacial acetic acid （gradient elution） at a flow rate of 1.0 ml/min. The detection wavelength was set at 206 nm. RESULTS： The linear range of loureirin B was 0.40-20.00 μg/ml（r=0.999 5）, respectively. RSDs of precision, stability and reproducibility tests were all lower than 2%. The average recoveries was 100.50% （RSD=1.90%, n=6）. CONCLUSIONS： The improved method is simple, well-separated, sensitive and reproducible, and can be used for the quality control of Liaoyuan qili controlled-release pellets. |
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| Keywords: | Liaoyuan qili controlled-release pellets Loureirin B HPLC Content determination |
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