HPLC法同时测定追风舒经活血片中4种成分的含量

目的:建立同时测定追风舒经活血片中羌活醇、异欧前胡素、蛇床子素和二氢欧山芹醇当归酸酯含量的方法。方法:采用高效液相色谱法。色谱柱为Hypersil C18(200 mm×4.6 mm,5μm),流动相为乙腈-0.05%冰醋酸溶液(梯度洗脱),流速为0.8 ml/min,检测波长为310 nm(羌活醇和异欧前胡素)、330 nm(蛇床子素和二氢欧山芹醇当归酸酯)。结果:羌活醇、异欧前胡素、蛇床子素和二氢欧山芹醇当归酸酯的线性范围分别为0.027 8⁰.556 0μg(r=0.999 5)、0.018 40.556 0μg(r=0.999 5)、0.018 4⁰.368 0μg(r=0.999 4)、0.059 20.368 0μg(r=0.999 4)、0.059 2¹.184 0μg(r=0.999 8)、0.012 61.184 0μg(r=0.999 8)、0.012 6⁰.252 0μg(r=0.999 3);四者精密度、稳定性、重复性试验的RSD<1%;平均加样回收率分别为98.69%(RSD=0.86%,n=6)、96.92%(RSD=0.62%,n=6)、99.13%(RSD=0.95%,n=6)、97.79%(RSD=1.38%,n=6)。结论:该方法准确、灵敏、重复性好,可用于追风舒经活血片的质量控制。

Simultaneous Determination of 4 Components in Zhuifeng Shujing Huoxue Tablet by HPLC

OBJECTIVE： To develop the method for the simultaneous determination of notopterol, isoimperatorin, osthol and columbianadin in Zhuifeng shujing huoxue tablet. METHODS：HPLC method was adopted. The determination was performed on Hypersil C J8 （200 mm×4.6mm,5μm） column with mobile phase consisted of acetonitrile-0.05% acetic acid solution （gradient elution） at the flow rate of 0.8 ml/min. The detection wavelength was set at 310 nm （notopterol and isoimperatorin） and 330 nm （osthole and columbianadin）. RESULTS：The linear ranges were 0.027 8-0.556 0 μg（r=0.999 5） for notopterol, 0.018 4-0.368 0μg for isoimperatorin （r=0.999 4）, 0.059 2-1.184 0μg for osthol （r=0.999 8） and 0.012 6-0.2520/ag for columbianadin （r=0.999 3）, respectively. RSDs of precision, stability and reproducibility tests were all lower than 1%. The average recoveries were 98.69% （RSD=0.86%, n=6）, 96.92% （RSD：0.62%, n=6）, 99.13% （RSD=0.95%, n=6） and 97.79% （RSD= 1.38%, n=6）. CONCLUSIONS ： The method is accurate, sensitive and reproducible, and can be used for the quality control of Zhuifeng shujing huoxue tablet.