木蝴蝶药材的质量控制方法研究

目的:建立木蝴蝶药材的质量控制方法。方法:按照2010年版《中国药典》(一部)方法测定不同产地木蝴蝶药材中的灰分、酸不溶性灰分与杂质的含量;采用高效液相色谱法测定药材中木蝴蝶苷B的含量:色谱柱为Promosil C18(200 mm×4.6 mm,5μm),流动相为甲醇-水-磷酸(42∶58∶0.2,V/V/V),流速为1.0 ml/min,柱温为25℃,检测波长为254 nm。结果:4个不同产地木蝴蝶药材的总灰分百分含量范围为2.16%³.99%;酸不溶性灰分范围为0.01%3.99%;酸不溶性灰分范围为0.01%⁰.86%;杂质范围为0.26%0.86%;杂质范围为0.26%⁰.52%;木蝴蝶苷B质量分数范围为4.49%0.52%;木蝴蝶苷B质量分数范围为4.49%⁶.14%。结论:所建立方法可用于木蝴蝶药材的质量控制。

Methods for Quality Control of Oroxylum indicum

OBJECTIVE： To establish the methods for quality control of Oroxylum indicum. METHODS ： The contents of ash, acid-insoluble ash and impurities in O. indicum from different producing areas were determined according to Chinese Pharmacopoeia （2010 edition）. The content of oroxin B was detected by HPLC. The determination was performed on Promosil C18 （200 mm× 4.6 mm, 5 μm） column with mobile phase consisted of methanol-water-phosphoric acid （42 ： 58 ： 0.2, V/V/V） at the flow rate of 1.0 ml/ min. The column temperature was 25 ℃, and the detection wavelength was set at 254 nm. RESULTS ： The contents of total ash in 0. indicum from 4 producing areas were between 2.16% and 3.99%; the contents of acid-insoluble ash were between 0.01% and 0.86%; the contents of impurities were between 0.26% and 0.52%; the contents of oroxin B were between 4.49% and 6.14%. CONCLUSIONS ： The method can be used for the quality control of O. indicum.