参红补血颗粒调控造血因子的作用研究

目的:研究参红补血颗粒调控造血因子的作用。方法:采用皮下注射乙酰苯肼,同时腹腔注射环磷酰胺以复制小鼠血虚证模型。60只ICR小鼠随机均分为正常对照(等容生理盐水)组、模型(等容生理盐水)组、复方阿胶(11 ml/kg)组与参红补血颗粒高、中、低剂量(9、6、3 g/kg)组,灌胃给药,每天1次,连续14 d。酶联免疫法检测小鼠血清粒细胞集落刺激因子(G-CSF)、巨噬细胞集落刺激因子(M-CSF)、可溶性黏附分子(VCAM-1)含量。结果:与正常对照组比较,模型组小鼠血清G-CSF、M-CSF、VCAM-1含量减少,差异有统计学意义(P<0.01或P<0.05);与模型组比较,参红补血颗粒高、中剂量组小鼠血清G-CSF含量增加,参红补血颗粒高、低剂量组小鼠血清M-CSF含量增加,参红补血颗粒高、中、低剂量组小鼠血清VCAM-1含量增加,差异有统计学意义(P<0.01或P<0.05)。结论:参红补血颗粒改善血虚证模型小鼠的造血功能可能与增加造血功能相关的细胞因子的含量,及调整模型小鼠的造血微环境状态有关。

Effects of Shenhong Buxue Granules on the Regulation of Hematopoietic Factor

OBJECTIVE： To study the effects of Shenhong buxue granules on the regulation of hematopoietic factor and hematopoietic microenvironment. METHODS： Blood deficiency syndrome model was induced by hypodermic injection of acetylphenyl- hydrazine and intraperitoneal injection of cyclophosphamide. 60 ICR mice were randomly divided into normal control group （constant volume of distilled water）, model group （constant volume of distilled water）, Compound ejiao syrup group （11 ml/kg） and Shenhong buxue granules high-dose, medium-dose and low-dose groups （9, 6, 3 g/kg）. They were given medicine intragastrically once a day for consecutive 14 days. The effects of Shenhong buxue granules on G-CSF and M-CSF, and the content of VCAM-1 were detected by ELISA. RESULTS： Compared with normal control group, the serum contents of G-CSF, M-CSF and VCAM-1 were decreased in model group, there was statistical significance （P〈0.01） ; compared with model group, the serum contents of G-CSF were increased in shenhong buxue granules high-dose and medium-dose groups, the serum contents of M-CSF were in- creased in shenhong buxue granules high-dose and low-dose groups, the serum contents of VCAM-1 were increased in shenhong buxue granules high-dose , medium-dose and low-dose groups, there was statistical significance （P〈O.O1, P-〈0.05）. CONCLU- SIONS： Shenhong buxue granules improve hematopoietic function of blood deficiency syndrome model mice, which may be associ- ated with the increase of the content of hematopoietic function related cytokines and the regulation of hematopoietic microenvironment in model mice.