白花丹参及其提取物中总丹酚酸含量测定方法研究

目的：建立并测定白花丹参及其提取物中总丹酚酸的含量。方法：采用亚硝酸钠-硝酸铝配位显色法，以供试品溶液和对照品溶液紫外-可见吸收图谱一致性为依据，比较丹酚酸B、丹参素钠和原儿茶醛作对照品的合理性；对亚硝酸钠-硝酸铝配位显色法中各显色剂加入量、加入显色剂后放置时间进行了考察，进行方法学考察。结果：丹酚酸B更适合作对照品，最佳显色条件为：精密加入1%NaNO₂ 2.5 mL，暗处放置10 min，再精密加入20%Al（NO₃）₃ 0.25 mL，暗处放置10 min，再精密加入1 mol·L^-1 NaOH 4 mL，暗处放置15 min。丹酚酸B在0.028~0.309 mg线性关系良好（r=0.999 9），白花丹参和提取物的回收率分别为96.29%，99.53%。5个批次白花丹参总丹酚酸的含量在5.65%~6.19%，提取物中总丹酚酸的含量在61.03%~61.96%。结论：建立了亚硝酸钠-硝酸铝配位显色法测定白花丹参及其提取物中总丹酚酸含量的方法。

Study on Method for Determining Content of Total Salvianolic Acids in the Root of Salvia miltiorrhiza alba and its Extration

Objective: To establish a method for determining content of total salvianolic acids in the root of Salvia miltiorrhiza alba(Baihua Danshen)and its extraction. Method: Based on the consistency of the characteristic adsorption bands in the visible absorption spectum between reference substance and sample solution of Baihua Danshen,salvianolic acid B,tanshion sodium and protocatechuic aldehyde were compared using NaNO₂-Al(NO₃)₃ colorimetric method.The best color conditions,which regarded the dosage of color developing agent and placing time in the dark after adding color developing agent as observation factors,were optimized and its methodology was investigated. Result: Salvianolic acid B was selected as the reference substance. The optimized color condition was as follows:precisely add 2.5 mL 1% NaNO₂ and place in the dark for 10 min,then accurately add 20%Al (NO₃)₃ 0.25 mL and place in the dark for 10 min,and lastly,precisely add 1 mol·L^-1 NaOH 4 mL and place in the dark for 15 min.The linear range was 0.028-0.309 mg(r=0.999 9) and the average recovery was 96.29% and 99.53% for Baihua Danshen and its extraction respectively. The content of total salvianolic acids in five batches of Baihua Danshen was ranged from 5.65% to 6.19% and in the extrations was ranged from 61.03% to 61.96%. Conclusion: The NaNO₂-Al(NO₃)₃ color imetric method for determining content of total salvianolic acids in Baihua Danshen and its extrationis established.