基于析因设计考察黄芪葛根汤配伍对糖尿病大鼠肝组织IL-12,IL-15的影响及其交互关系

目的：考察黄芪葛根汤对糖尿病大鼠血糖和肝组织胆固醇（CHO）、甘油三酯（TG）及白介素-12（IL-12）、白介素-15（IL-15）的影响，分析黄芪、葛根在调节糖尿病过程中的交互关系。方法：将66只大鼠按血糖随机分为6组，每组11只；正常组，模型组（A1B1），阳性对照金芪降糖片组，黄芪组（A2B1），葛根组（A1B2），黄芪葛根汤组（A2B2）。按46 mg·kg^-1剂量给SD大鼠1次ip STZ诱导糖尿病模型，除正常组、阳性对照组外，其余各组按2×2析因设计实施实验。阳性对照金芪降糖片组按1.47 g·kg^-1 ig，黄芪组、葛根组、黄芪葛根汤组分别按2.7，1.35，4.05 g·kg^-1 ig。治疗30d测定随机血糖和血清CHO，TG与肝组织IL-12，IL-15等指标。有关联的指标计算加权综合评分，对综合指标运用析因设计方差分析方法分析黄芪、葛根的主效应及其交互效应。结果：糖尿病模型大鼠血糖、血清CHO，TG与肝组织IL-12，IL-15水平均较正常组升高（P<0.05）。黄芪、葛根、黄芪葛根汤均可下调血糖与血清CHO，TG（黄芪葛根汤组除外）及肝组织IL-12，IL-15水平（P<0.05）。结论：葛根降低血糖作用佳，与黄芪无交互作用，黄芪葛根汤在下调血糖方面未呈现药物配伍的叠加效应；在综合调节血清CHO，TG及肝组织IL-12，IL-15水平方面，黄芪葛根二药配伍合用优于单味药。

Based on Factorial Design to Investigate Compatibility Effect of Huangqi Gegen Decoction on Liver Tissue of Diabetes Rats with IL-12,IL-15 and its Interaction Relationship

Objective: To observe the compatibility of Huangqi Gegen decoction root have impact on blood glucose, blood lipid and interleukin-12(IL-12),interleukin-15(IL-15) in rats with diabetes mellitus (DM),and regulate the interaction between diabetes processes. Method: Sixty-six SD rats were randomly divided into 6 groups, normal group, model group (A1B1), positive control group, Astragali Radix group (A2B1), Puerariaelobatae Radix group (A1B2),Huangqi Gegen decoction group (A2B2),11 each group. In addition to the normal group and positive control group,the rest of the group were induced by Streptozotocin(STZ,46 mg·kg^-1) made after the model according to the 2×2 factorial design experiment scheme. Positive control group by 1.47 g·kg^-1 dose gavage, Astragali Radix group, Puerariaelobatae Radix group,Huangqi Gegen decoction group were 2.7 g·kg^-1, 1.35 g·kg^-1,4.05 g·kg^-1 dose ig 30 days.Blood lipid and the levels of IL-12 and IL-15 in liver of rats indicators, etc.Were detected related index to calculate the weighted composite score of comprehensive index using the factorial design analysis of variance method study astragalus and kudzu root main effect and interaction effect. Result: Compared with normal group, blood glucose,serum cholesterol, triglyceride, IL-12, IL-15 in liver of diabetes rats were increased significantly(P<0.05).Astragali Radix, Puerariaelobatae Radix and Huangqi Gegen decoction effectively reduce blood glucose, serum cholesterol, triglyceride(in addition to Huangqi Gegen decoctio), IL-12 and IL-15(P<0.05). Conclusion: Puerariaelobatae Radix lowering blood sugar is good, no interaction with Astragali Radix, Huangqi Gegen decoction additive effect in terms of lowered blood sugar drug compatibility is not presented; comprehensive regulation in liver tissue cholesterol, triglyceride, concentration of IL-12 and IL-15, Astragali Radix and Puerariaelobatae Radix compatibility of the two drugs in combination is superior to single herbs.