多指标正交试验法优选桂圆益气补血汤煎煮工艺

目的：优选桂圆益气补血汤的煎煮工艺条件。方法：采用HPLC测定阿魏酸、黄芪甲苷含量，流动相分别为甲醇-0.05%磷酸溶液（60：40），乙腈-水（33：67），检测波长分别为320，203 nm。以干膏得率和黄芪甲苷、阿魏酸、总多糖含量的综 合评分为指标，通过正交试验考察煎煮时间、料液比、煎煮次数对桂圆益气补血汤煎煮工艺的影响。结果：最佳煎煮工艺条件为加12倍量水煎煮3次，每次36 min，煎煮前浸泡30 min；浸膏得率39.3%，阿魏酸、黄芪甲苷提取量分别为18.24，68.17 mg·g^-1，多糖得率6.80%。结论：优选的煎煮工艺条件稳定且重复性好，适用于桂圆益气补血汤的工业生产。

Optimization of Decocting Technology for Guiyuan Yiqi Buxue Decoction by Multi-index Orthogonal Design

Objective: To optimize decocting technology conditions of Guiyuan Yiqi Buxue decoction. Method: HPLC was adopted to determine contents of ferulic acid and astragaloside with mobile phases of methanol-0.05% phosphoric acid(60:40) and acetonitrile-water(33:67),detection wavelength of 320 and 203 nm,respectively.Taking dry extract yield,contents of astragaloside and ferulic acid,yield of total polysacchrides as comprehensive evalutaion index,orthogonal test was used to optimize decoction conditions with solid-liquid ratio,decoction time and decoction times as factors. Result: Optimum decoction conditions were as follows:decocted 3 times with 12-fold water,36 minutes each time,soaked 30 min before decocting;Under these conditions,dry extract yield was 6.80%,extraction amount of astragaloside and ferulic acid were 68.17 and 18.24 mg·g^-1,yield of total polysacchrides was 6.80%. Conclusion: This optimized decoction technology was stable and reproducible,which was suitable for industrial production of Guiyuan Yiqi Buxue decoction.