哮喘小鼠C7-T5节段脊髓后角PKD1与PKD2的表达研究

目的探讨在哮喘发病中，蛋白激酶D1、蛋白激酶D2（proteinkinaseD1，D2，PKD1，PKD2）在哮喘小鼠c7-T5节段脊髓后角内的表达变化。方法BALB／c小鼠20只，按随机数字表法均分为正常对照组和哮喘组，利用AniRes2005肺功能仪测小鼠气道阻力、免疫荧光方法和Westernblot方法检测各组小鼠c7-T5节段脊髓后角内PKD1，PKD2的表达变化。结果哮喘组小鼠吸气阻力和呼气阻力明显高于正常组（P〈0．01），哮喘模型建立成功。免疫荧光结果显示哮喘组c7．T5节段脊髓后角内PKD1，PKD2阳性产物的平均光密度值（MOD）显著高于正常对照组（P〈0．01），westernblot方法检测也得到了相同的结果。结论哮喘小鼠C7-T5节段脊髓后角内PKD1，PKD2的表达升高。

The expression of PKD1 ,PKD2 in the spinal dorsal born of the asthmatic mice

Objective To study the expression of protein kinase D1 and protein kinase D2 in the spinal dorsal horn of the asthmatic mice. Methods 20 BALB/e mice were randomly divided into normal control group and asthmatic group. The airway re- sistance of mice was measured with AniRes 2005 pulmonary function meter; The expressions of PKD1 and PKD2 were observed by immunofluoreseence and western blot methods of the both groups. Results Inspiratory resistance and expiratory resistance in the asthmatic mice were obviously higher than in normal control mice, the asthmatic model was successfully constructed. The mean optic density （MOD） of PKD1 and PKD2 positive product in the C7-T5 spinal dorsal horn increased significantly in the asthmatic mice than in normal control by means of immunofluorescence （P〈0.01）, and the same result was obtain by western blot method. Conclusion The expressions of PKD1 and PKD2 were elevated in the C7-T5 spinal dorsal hom of the asthmatic mice.