| 睾丸支持细胞促进体外培养神经元生长的研究 |
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| 引用本文： | 上官芳芳,史小林. 睾丸支持细胞促进体外培养神经元生长的研究[J]. 解剖学报, 2003, 34(5): 510-513 |
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| 作者姓名： | 上官芳芳 史小林 |
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| 作者单位： | 首都医科大学生殖医学研究中心,北京,100054;首都医科大学生殖医学研究中心,北京,100054 |
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| 摘 要： | 目的 探讨睾丸支持细胞 (SC)对体外培养神经元生长发育的促进作用。 方法 将SCs制备成饲养层和SCs条件培养液 (SCM)后与神经元共培养 ,观察培养细胞的存活数和突起数 ,并应用图像分析仪观察细胞的面积。 结果 SCs饲养层及SCM培养神经元的存活数、突起数和胞体面积明显高于对照组 (P <0 0 1)。 结论SCs对体外培养的神经元具有显著的促生长作用。
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| 关 键 词： | 睾丸支持细胞 细胞培养 神经元 |
THE PROMOTIVE EFFECT OF SERTOLI CELL ON NEURONS in vitro |
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| SHANGGUAN Fan-fang,SHI Xiao-lin. THE PROMOTIVE EFFECT OF SERTOLI CELL ON NEURONS in vitro[J]. Acta Anatomica Sinica, 2003, 34(5): 510-513 |
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| Authors: | SHANGGUAN Fan-fang SHI Xiao-lin |
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| Affiliation: | SHANGGUAN Fan-fang,SHI Xiao-lin * |
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| Abstract: | Objective To study the promotive effect of SCs on neurons in vitro Methods SCs feeder layer was made as conditioned medium(SCM), then used in coculture with neurons respectively. Count the survived neurites of the cultured cell and observe the cell size by a medical image analyzer. Results The number of surviving cells, neurites outgrowth and cell size cocultured either with SCs feeder layer or SCM was markedly increased than that of control cultures(P<0.01).Conclusion SCs exert a promotive effect on the growth of neurons in vitro |
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| Keywords: | Sertoli cells Cell culture Neurons |
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