乙型肝炎病毒DNA聚合酶活力测定及临床意义

采用特异性免疫沉淀法检测血清中HBV DNAP活力216例,结果:HBeAg和抗-HBcIgM阳性组的HBV DNAP检出率和活力水平明显高于阴性组(P<0.01);且随HBsAg的滴度升高而升高。在各类乙型肝炎病人中DNAP检出率依次为;重症肝炎和急性肝炎>肝硬化和慢性潘动性肝炎>慢性迁延性肝炎和携带者。抗-HBe阳性组和HBeAg、抗-HBe均阴性组各有25％、54.71％可测到DNAP活力。证明HBV DNAP活力测定是乙型肝炎早期诊断、病毒在体内复制及是否具有传染性的灵敏指标。

DETERMINATION OF HEPATITIS B VIRUS DNA POLYMERASE AND ITS CLINICAL SIGNIFICANCE

HBV DNA polymerase in serum was determined by immunoprecipitation method. We examined 216 serum samples from hepatitis suffers and carriers. The result suggested that the rate of positive HBV DNAP and the level of activity of HBV DNAP in HBeAg and anti-HBcIgM positive groups were both significantly higher than those of negative group (P<0.01), and increase in titers of HBsAg was followed by subsequent increase of positive rate and level of activity. DNAP potitive rates in all types of hepatitis B occurred successively as: serious hepatitis and acute hepatitis cirrhosis of liver and chronic active hepatitis chronic persistent hepatitis and carriers. In positive group of anti-HBe and both negative groups of HBeAg and anti-HBe, the activity of DNAP were detected in 25% and 54.71% of cases respectively. Above results revealed that measurement of activity of HBV DNAP serves as a sensitive clue to early diagnosis of hepatitis B, and an indicator for the occurrence of replication of virus and its infectivity.