脐血干细胞体外程序扩增的实验研究

目的 利用一种新的体外扩增脐带血原始细胞的方法，使得干细胞在数量增加的同时，又能尽量保持原始性。方法 在相同细胞因子组合下，用体外液体培养比较脐血有核细胞程序扩增组与一次扩增组及有无基质层条件下的优劣。结果 培养28d后，虽然程序扩增组MNC细胞绝对数低于一次扩增组，但其CD34 细胞数明显高于一次扩增组，且有基质层组细胞总数，原始细胞数均高于无基层组，结论 体外程序扩增脐带干细胞是一种有效的扩增干细胞的方法，从而使得脐血的应用更为广泛。

Experimental study of sequential expansion of umbilical blood stem cells in vitro

Objective Using a new method to expand the number of umbilical cord blood stem cells culture in vitro, at the same time to keep their originality. Methods under the same condition with hematopoietic factors in the liquid culture, compared the sequential expansion group and once for all expansion group with or without stroma celllayer. Results When cultured for 28 days, the absolute number of MNC cells in the sequential expansion group was lessthan that in the once for all expansion group,but the number of CD34 + cell in the former was more than that in thelatter. Furthermore, the total number of MNC and progcnitor were greater in that with presence of stromal cell layerthan that with sbsence of stromal cell layer. Conclusion Sequential expansion of umbilical blood stem cells is an effective method for expansion of srem cells which enables more extensive use of umbilical blood. (Shanghai Med J, 2001,24: 95-98)