柴胡三种萃取物抗惊厥作用有效成分探讨

探讨柴胡三种萃取物抗惊厥作用的有效成分。采用气相色谱-质谱联用仪(GC-MS)及高效液相色谱仪(HPLC)分析超临界CO_2萃取(SFE-CO_2)获得的柴胡皂甙部分、挥发油部分及水溶性部分三者的化学成分,共鉴定出40种化学成分,其中,对最大电休克(MES)电惊厥模型有明显拮抗作用的柴胡皂甙部分及挥发油部分,它们的挥发性化学成分都以亚油酸为主,而能有效对抗戊四唑(PTZ)化学惊厥模型的水溶性部分,它的挥发性化学成分则主要为丙三醇和木糖醇,故推测柴胡所含的亚油酸可能有抗电惊厥作用,所含的两种醇可能对化学性惊厥起拮抗作用。

Chemical Constituents Analysis on Anticonvulsive Effect of Three Extracts from Radix Bupleuri

Objective: To study the relationship between anticonvulsive effect of three extracts from Radix Bupleuri and their chemical compositions. Methods: Volatile oil component, saikosaponin component and water-solutble component were extracted with SFE-CO2 from Radix Bupleuri, their effect on MES and PTZ seizure models had been studied, and their compositions were analyzed by GC-MS and HPLC. Results: Compared with the control, volatile oil component and saikosaponin component significandy decreased anticonvulsive occurrence on MES, the main chemical compositions of them was linoleic acid; water-solutble component significantly decreased anticonvulsive occurrence on PTZ seizure, but most of its compositions were glycerol and xylitol. Conclusion: Linoleic acid from Radix Bupleuri maybe fight against MES seizure, while glycerol and xylitol could fight against PTZ seizure.