超速玻璃化冷冻载体Cryotop和Cryoloop冻存小鼠卵裂期胚胎的效果比较

目的应用冷冻薄膜(Cryotop)和冷冻环(Cryoloop)两种超速玻璃化冷冻载体,氯化胆碱替代氯化钠的低钠磷酸盐缓冲液配置的玻璃化冷冻保护剂冷冻小鼠胚胎,比较两种载体的效果差异。方法将300个小鼠胚胎随机分为3组:对照组60个、Cryotop组120个和Cryoloop组120个。比较复苏后胚胎存活率和继续发育能力。结果 Cryotop组复苏后胚胎存活率达93.3%,Cryoloop组90.4%,无显著性差异(P>0.05);继续发育率:Cryotop组和Cryoloop组比较,4细胞率分别为48.2%和29.8%(P<0.05),囊胚率分别为31.5%和16.1%(P<0.05),差异具有显著性。结论Cryotop和Cryoloop两种玻璃化冷冻载体相比,Cryotop操作更为简便,复苏后小鼠胚胎获得了更高的继续发育能力。

Comparison of two ultra-rapid vitrification carriers for mouse two-cell embryos： cryotop and cryoloop

Objective： To compare the results of Cryotop and Cryoloop vitrification carriers for the purpose of establishing an effective ultra-rapid vitrification method for cleavage embryos.Methods： The 2-cell mouse embryos were divided into three groups randomly,60 cells in control group,120 cells in cryotop group and 120 cells in Cryoloop group.At first the survival rates were evaluated through the embryo morphology after thawing.Then the survival embryos were evaluated by continue development rates.Results： There was no difference in embryo survival rates between cryoloop group and Cryotop group.Compared to cryoloop group,there was significant higher continue development rates in the Cryotop group.Conclusion： The cryotop ultra-rapid vitrification technique not only obtained higher embryo survival rate but also conserved the embryos vitality.It is more effective to cryopreserve early cleavage embryos.